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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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AIO-Puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_74630 | Ampicillin | PMID:27079678 | Puromycin resistance gene is only for transient expression along with Cas9 nickase. Thus, drug selection period should be tested for each experiment. The presence of cellular puromycin resistance could be monitored by performing a control transfection with an empty backbone vector of either AIO-GFP or AIO-mCherry in which GFP/mCherry expression level represents puromycin resistance level. | Backbone Size:9626; Vector Backbone:All-in-One_Nickase-D10A; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 7 | |||
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pFM571 Resource Report Resource Website |
RRID:Addgene_74634 | mCherry | Ampicillin | PMID:26994102 | During verification of the constructed cassettes by restriction analysis and sequencing we found out that there are 254 bp missing after the BamHI site in between the fluorophore and the Ashbya gossypii TEF promotor region regulating expression of the selectable marker in pFM571, pIM572 and pIM573. We checked the original pYM27 plasmid (Janke, et al., 2004, Yeast 21:947-962 ) we had in our hands and compared the obtained sequence to the published one. We also checked by restriction analysis pYM27 from an independent source (but also obtained from Euroscarf) and got the same results. In the published pYM27 sequence, there is a sequence AGATCC resembling BamHI site (GGATCC) at position 1044-1049 bp. Likely due to a BamHI star activity that led to cutting this BamHI-like site in parallel with the true BamHI site, the nucleotides between these two sites got lost during construction of pYM27 or its predecessors and are missing in pYM27 and thus also in our pYM27-based modules - pFM571, pIM572 and pIM573. It seems, however, that the functionality of the vector and of the selection marker is not disturbed since transformants were readily obtained with all the concerned cassettes when selecting on geneticin. | Backbone Marker:Janke et al., (2004) Yeast 21:947-962; Backbone Size:4940; Vector Backbone:pYM27; Vector Types:; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 0 | ||
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pGEX6P1-human LIC1 C-terminal half Resource Report Resource Website 1+ mentions |
RRID:Addgene_74599 | human dynein light intermediate chain 1 amino acids 389-523 | Homo sapiens | Ampicillin | PMID:25272277 | Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | amino acids 389-523 of LIC1 | Addgene | 2026-10-10 01:57:43 | 1 | |
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pIM700 Resource Report Resource Website |
RRID:Addgene_74639 | yTagRFP-T | Ampicillin | PMID:26994102 | Backbone Marker:Marian Farkasovsky; Backbone Size:4522; Vector Backbone:pFM699; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 0 | |||
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pIM690 Resource Report Resource Website |
RRID:Addgene_74638 | yTagRFP-T | Ampicillin | PMID:26994102 | Compared to the published sequence of the pFA6a-hphNT1 backbone vector (Janke, et al., 2004, Yeast 21: 947-962) we found one PstI site missing in its sequence. It is the site in the vector –promoter part and there is a substitution of A by G in position 284 bp that arose most probably during construction of preceding cassettes, e.g. pFA6-hphMX4. We introduced the substitution also into maps of our modules carrying the hygromycin resistance - pFM689, pIM690 and pIM691. | Backbone Marker:Marian Farkasovsky; Backbone Size:4905; Vector Backbone:pFM689; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 0 | ||
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pFM699 Resource Report Resource Website |
RRID:Addgene_74636 | mCherry | Ampicillin | PMID:26994102 | Backbone Marker:Janke et al., (2004) Yeast 21:947-962; Backbone Size:3784; Vector Backbone:pFA6a-natNT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 0 | |||
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pcFLAG-CSK-R107E Resource Report Resource Website |
RRID:Addgene_74503 | CSK | Homo sapiens | Ampicillin | PMID:23707526 | The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function. | Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R107E, K222R | Addgene | 2026-10-10 01:57:43 | 0 |
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pKHR7 Resource Report Resource Website |
RRID:Addgene_74624 | cmlc2::mCherry | Danio rerio | Ampicillin | PMID:27003937 | Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 0 | ||
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pcFLAG-CSK-WT Resource Report Resource Website |
RRID:Addgene_74502 | CSK | Homo sapiens | Ampicillin | PMID:23707526 | The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function. | Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K222R | Addgene | 2026-10-10 01:57:43 | 0 |
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pcDNA3-FLAG-Grb10-WT Resource Report Resource Website |
RRID:Addgene_74501 | Grb10 | Homo sapiens | Ampicillin | PMID:23246379 | Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | L358S, V522A, and V524L | Addgene | 2026-10-10 01:57:43 | 0 | |
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pCR3.1-mIL2 Resource Report Resource Website |
RRID:Addgene_74467 | IL2 | Mus musculus | Ampicillin | PMID:11198480 | Backbone Marker:Invitrogen; Backbone Size:5060; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:43 | 0 | ||
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pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids) Resource Report Resource Website |
RRID:Addgene_74505 | CSK | Homo sapiens | Ampicillin | PMID:23707526 | The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function. | Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | SH3-SH2 domains | Addgene | 2026-10-10 01:57:43 | 0 |
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pcFLAG-CSK-delta SH3 (73 to450 amino acids) Resource Report Resource Website |
RRID:Addgene_74504 | CSK | Homo sapiens | Ampicillin | PMID:23707526 | Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | del-SH3 (73 to450 amino acids) | Addgene | 2026-10-10 01:57:43 | 0 | |
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pKHR8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_74625 | FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette | Danio rerio | Ampicillin | PMID:27003937 | Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Cre/Lox, Zebrafish Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:44 | 2 | ||
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pMSCVneo-HOXB3-FLAG Resource Report Resource Website |
RRID:Addgene_74508 | HOXB3 | Homo sapiens | Ampicillin | PMID:26482852 | Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:43 | 0 | ||
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NPY-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_74629 | Neuropeptide Y | Homo sapiens | Kanamycin | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:GFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-10 01:57:44 | 5 | |||
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pKF297 Resource Report Resource Website |
RRID:Addgene_74774 | TEV- 2xV5 tag (short version) | synthetic | Ampicillin | PMID:27172193 | We observe rather poor growth of bacteria with the pJet1.2 backbone, resulting in low yields of the plasmid preps. | Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:45 | 0 | |
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BRD1A Resource Report Resource Website |
RRID:Addgene_74655 | BRD1A | Homo sapiens | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|11321642 | Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-10 01:57:44 | 0 | ||
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MSCV-dGFP-JARID1C Resource Report Resource Website |
RRID:Addgene_74776 | JARID1C | Homo sapiens | Ampicillin | PMID:25712104 | GFP is driven by a separate promoter (UBC promoter), and is used only to track cells and the efficiency of infection/transfection. | Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-10 01:57:45 | 0 | |
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STK10 Resource Report Resource Website |
RRID:Addgene_74659 | STK10 | Homo sapiens | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|5174701 | Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-10 01:57:44 | 0 |
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