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Species: Synthetic
Genetic Insert: OsU6.2-sgRNA7 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199733 Copy
Species: Synthetic
Genetic Insert: TaU6-sgRNA8 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199734 Copy
Species: Synthetic
Genetic Insert: ZmU6C3-sgRNA5 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199731 Copy
Species: Synthetic
Genetic Insert: ZmU6C1-sgRNA4 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199730 Copy
Species: Synthetic
Genetic Insert: Expression unit for PuroR.T2A.EGFP selectable and reporter markers
Vector Backbone Description: Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237).
Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting.
Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease.
Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199227 Copy
Species: Synthetic
Genetic Insert: Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene at the human CCR5 safe harbor locus when delivered together with gRNA expression plasmid AY22_pgRNA.R5.1 (Addgene plasmid #100294) and a SpCas9 nuclease (HMEJ-based gene targeting) or a SpCas9.D10A nickase (ITPN-based gene targeting).
Note: to reduce the chances for off-targeting donor DNA insertions when applying HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199223 Copy
Species: Synthetic
Genetic Insert: OsU3-sgRNA2 scaffold
Vector Backbone Description: Vector Backbone:pYPQ132D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199728 Copy
Species: Synthetic
Genetic Insert: TaU3-sgRNA3 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199729 Copy
Species: Synthetic
Genetic Insert: sgRNA scaffold
Vector Backbone Description: Vector Backbone:pYPQ141D; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199726 Copy
Species: Synthetic
Genetic Insert: Multiple Cloning Site, NPTII
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199724 Copy
Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199722 Copy
Species: Synthetic
Genetic Insert: 35S-RUBY
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Comments: Addgene sequencing identified partial duplication of the CaMV 35S enhancer element. Depositing lab has confirmed that the duplication was present in their original stock of the plasmid and does not disrupt RUBY function.
Proper citation: RRID:Addgene_199723 Copy
Species: Synthetic
Genetic Insert: DARPin-hFc 27F3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-855-2309-D7
Proper citation: RRID:Addgene_199619 Copy
Species: Synthetic
Genetic Insert: DARPin-FLAG 27G2
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-855-2308-A9
Proper citation: RRID:Addgene_199616 Copy
Species: Synthetic
Genetic Insert: DARPin-hFc E3_5 (control)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-782-2400-H3
Proper citation: RRID:Addgene_199617 Copy
Species: Synthetic
Genetic Insert: DARPin-FLAG E3_5 (control)
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-782-2400-H3
Proper citation: RRID:Addgene_199612 Copy
Species: Synthetic
Genetic Insert: PEmax-P2A-BSD
Vector Backbone Description: Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37119812
Proper citation: RRID:Addgene_191102 Copy
Species: Synthetic
Genetic Insert: NRCH-PE2-BSD
Vector Backbone Description: Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37119812
Proper citation: RRID:Addgene_191101 Copy
Species: Synthetic
Genetic Insert: Expression unit for EGFP reporter
Vector Backbone Description: Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237).
Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting.
Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease.
Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199228 Copy
Species: Synthetic
Genetic Insert: hTfR-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37098770
Comments: Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.
Proper citation: RRID:Addgene_199714 Copy
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