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Genetic Insert: FKBP-linker-Myc-NBirA*
Vector Backbone Description: Vector Backbone:pSF3; Vector Types:Mammalian Expression, Retroviral, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28585547
Proper citation: RRID:Addgene_90009 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Size:5137; Vector Backbone:pcDNA5 frt/to; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26735014
Proper citation: RRID:Addgene_90005 Copy
Species: Danio rerio
Genetic Insert: HA-BirA-2A-citrine
Vector Backbone Description: Backbone Size:4780; Vector Backbone:pGEM-GFP-SV40pA-FRT-Kan-FRT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666124
Proper citation: RRID:Addgene_89890 Copy
Species: Rattus norvegicus
Genetic Insert: Cacnb1
Vector Backbone Description: Backbone Size:5129; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27489103
Proper citation: RRID:Addgene_89893 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Comments: This Cas9 has been modified to contain the high-fidelity mutations for SpCas9-HF1 from Kleinstiver et al 2016
Proper citation: RRID:Addgene_89961 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pEM-Cas9HF1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89962 Copy
Genetic Insert: trmI gRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89955 Copy
Species: Mus musculus
Genetic Insert: F-actin-capping protein subunit alpha-1
Vector Backbone Description: Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26564775
Proper citation: RRID:Addgene_89950 Copy
Species: E. coli
Genetic Insert: LacI-11/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90059 Copy
Species: E. coli
Genetic Insert: LacI/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90058 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Claudia Schmidt-dannert; Backbone Size:2867; Vector Backbone:pACBB-eGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188969 Copy
Species: Bos taurus
Genetic Insert: GPCR activation based oxytocin sensor GRAB_OT1.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593404
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.02.10.480016v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_185386 Copy
Species: Mus musculus
Genetic Insert: RTN3C
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186963 Copy
Species: Homo sapiens
Genetic Insert: ARL6IP1
Vector Backbone Description: Backbone Size:5345; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35471903
Proper citation: RRID:Addgene_186965 Copy
Species: Multiple
Genetic Insert: dCas9-KRAB(Kox1)-MeCP2
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element. pCL51. Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188902 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U6 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173157 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:35998606
Proper citation: RRID:Addgene_188972 Copy
Species: Synthetic
Genetic Insert: synDNA 266
Vector Backbone Description: Backbone Marker:2710; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186185 Copy
Species: Synthetic
Genetic Insert: synDNA 246
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186183 Copy
Species: Synthetic
Genetic Insert: synDNA 236
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Synthetic Biology, Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36317886
Proper citation: RRID:Addgene_186182 Copy
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