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pCBLsgRNA7
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199733 OsU6.2-sgRNA7 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
pCBLsgRNA8
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199734 TaU6-sgRNA8 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
pCBLsgRNA5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199731 ZmU6C3-sgRNA5 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
pCBLsgRNA4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199730 ZmU6C1-sgRNA4 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
AD59_pEP.DonorCLYBL.TS
 
Resource Report
Resource Website 		RRID:Addgene_199227 Expression unit for PuroR.T2A.EGFP selectable and reporter markers Synthetic Ampicillin PMID:36928106 This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237). Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting. Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting. Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin 2026-09-01 09:35:53 0
BB44_pmc.DonorR5.TS
 
Resource Report
Resource Website 		RRID:Addgene_199223 Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004) Synthetic Ampicillin PMID:36928106 This target site-containing donor plasmid can be used to insert a transgene at the human CCR5 safe harbor locus when delivered together with gRNA expression plasmid AY22_pgRNA.R5.1 (Addgene plasmid #100294) and a SpCas9 nuclease (HMEJ-based gene targeting) or a SpCas9.D10A nickase (ITPN-based gene targeting). Note: to reduce the chances for off-targeting donor DNA insertions when applying HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) leads to seamless ITPN-mediated gene targeting. Backbone Size:2700; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin 2026-09-01 09:35:53 0
pCBLsgRNA2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199728 OsU3-sgRNA2 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ132D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
pCBLsgRNA3
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199729 TaU3-sgRNA3 scaffold Synthetic Tetracycline PMID:37070560 Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline 2026-09-01 09:36:01 1
pCBLgRNA
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199726 sgRNA scaffold Synthetic Spectinomycin PMID:37070560 Vector Backbone:pYPQ141D; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-09-01 09:36:01 1
pCBL101.1
 
Resource Report
Resource Website 		RRID:Addgene_199724 Multiple Cloning Site, NPTII Synthetic Spectinomycin PMID:37070560 Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin "GAGACC" to "GATACC" 2026-09-01 09:36:01 0
pCBL101
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199722 NPTII Synthetic Spectinomycin PMID:37070560 Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-09-01 09:36:01 1
pCBL101-RUBY
 
Resource Report
Resource Website 		RRID:Addgene_199723 35S-RUBY Synthetic Spectinomycin PMID:37070560 Addgene sequencing identified partial duplication of the CaMV 35S enhancer element. Depositing lab has confirmed that the duplication was present in their original stock of the plasmid and does not disrupt RUBY function. Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-09-01 09:36:01 0
pcDNA3.1_27F3_hFc
 
Resource Report
Resource Website 		RRID:Addgene_199619 DARPin-hFc 27F3 Synthetic Ampicillin PMID:36314779 HTC DARPin name 008-855-2309-D7 Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:35:59 0
pQIq_MRGS_HIS8_(27G2)_FLAG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199616 DARPin-FLAG 27G2 Synthetic Ampicillin PMID:36314779 HTC DARPin name 008-855-2308-A9 Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:35:59 1
pcDNA3.1_ E3_5 _hFc
 
Resource Report
Resource Website 		RRID:Addgene_199617 DARPin-hFc E3_5 (control) Synthetic Ampicillin PMID:36314779 HTC DARPin name 008-782-2400-H3 Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:35:59 0
pQIq_MRGS_HIS8_( E3_5)_FLAG
 
Resource Report
Resource Website 		RRID:Addgene_199612 DARPin-FLAG E3_5 (control) Synthetic Ampicillin PMID:36314779 HTC DARPin name 008-782-2400-H3 Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:35:59 0
pLenti-PE2max-BSD
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_191102 PEmax-P2A-BSD Synthetic Ampicillin PMID:37119812 Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin Changed R221K, N394K, H840A from SpCas9 2026-09-01 09:33:37 1
pLenti-NRCH-PE2-BSD
 
Resource Report
Resource Website 		RRID:Addgene_191101 NRCH-PE2-BSD Synthetic Ampicillin PMID:37119812 Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 09:33:37 0
AZ64_pE.DonorCLYBL.TS
 
Resource Report
Resource Website 		RRID:Addgene_199228 Expression unit for EGFP reporter Synthetic Ampicillin PMID:36928106 This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237). Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting. Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting. Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin 2026-09-01 09:35:53 0
pAAV_CMV::hTfR-GFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_199714 hTfR-GFP Synthetic Ampicillin PMID:37098770 Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids. Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-09-01 09:36:01 1

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