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Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168151 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168152 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168138 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST minimal CRISPR array (with spacer for AvPSP1)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168133 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST Tns proteins (TnsAB and TnsC)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168161 Copy
Species: Solanum lycopersicum
Genetic Insert: Tomato E8 promoter
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH41295; Vector Types:Synthetic Biology, MoClo Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34177999
Proper citation: RRID:Addgene_169088 Copy
Species: Beta vulgaris
Genetic Insert: BvDODA1
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH41308; Vector Types:Synthetic Biology, MoClo Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34177999
Proper citation: RRID:Addgene_169085 Copy
Species: Dorotheanthus bellidiformis
Genetic Insert: Db5GT
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH41308; Vector Types:Synthetic Biology, MoClo Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34177999
Proper citation: RRID:Addgene_169086 Copy
Species: Homo sapiens
Genetic Insert: VPS4B
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33326793
Proper citation: RRID:Addgene_169320 Copy
Species: Homo sapiens
Genetic Insert: VPS4A
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33326793
Proper citation: RRID:Addgene_169319 Copy
Genetic Insert: New vector
Vector Backbone Description: Vector Backbone:pSPL; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32610085
Comments: For a more detailed description, go to:
Furukawa, H, Simorowski, N, Michalski, K (January 2021) Effective production of oligomeric membrane proteins by EarlyBac-insect cell system. In: Methods in Enzymology. Elsevier.
DOI: 10.1016/bs.mie.2020.12.019
For online protocol, go to: http://furukawalab.labsites.cshl.edu/protocols/earlybac/
Proper citation: RRID:Addgene_170462 Copy
Genetic Insert: gRNA library targeting S. pneumoniae D39V genetic elements
Vector Backbone Description: Backbone Marker:Addgene 141090; Vector Backbone:pPEPZ-sgRNAclone; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33120116
Proper citation: RRID:Addgene_170432 Copy
Genetic Insert: Clover_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71900 Copy
Species: Synthetic
Genetic Insert: 4xNLS-PUFa-2xNLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71903 Copy
Species: Synthetic
Genetic Insert: 4xNLS-PUFb-2xNLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71904 Copy
Species: Synthetic
Genetic Insert: Clover_PUFc
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71901 Copy
Species: Synthetic
Genetic Insert: 4xNLS_PUFa_2xNLS_mCBPHAT
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71912 Copy
Species: Synthetic
Genetic Insert: mCBPHAT_4xNLS_PUFa_2xNLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71913 Copy
Species: Triticum aestivum
Genetic Insert: Promoter: U6 (Triticum aestivum)
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26616834
Proper citation: RRID:Addgene_68262 Copy
Species: Other
Genetic Insert: empty vector
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23669743
Proper citation: RRID:Addgene_68239 Copy
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