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Species: Synthetic
Genetic Insert: CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Vector Backbone Description: Backbone Marker:Andrew Gleave; Backbone Size:5042; Vector Backbone:pART7; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15086798
Comments: pART7::CLOMELEON is a pART7 vector with the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) integrated between EcoRI and HindIII restriction sites. See the Supplemental Document under Resource Information for more details, including annotated maps.
Proper citation: RRID:Addgene_247987 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41453699
Proper citation: RRID:Addgene_206873 Copy
Species: Synthetic
Genetic Insert: 3'UTR cloning site
Vector Backbone Description: Backbone Marker:Addgene #84771; Vector Backbone:BTV; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39014073
Proper citation: RRID:Addgene_213963 Copy
Species: Synthetic
Genetic Insert: NLS-NOS terminator-Hygromycin resistance
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pGGF000; Vector Types:GreenGate cloning entry vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246819 Copy
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pICH47742; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making full transcriptional unit modules in Level 1 position 2 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1092, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245699 Copy
Genetic Insert: MBP-His-TEV-ECOR31 Hachiman HamA
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39395413
Comments: Please visit https://doi.org/10.1101/2024.02.29.582594 for bioRxiv preprint.
Proper citation: RRID:Addgene_223369 Copy
Species: Homo sapiens
Genetic Insert: RECK
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV-9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304675
Comments: Gene sourced from Incyte cDNA clone LIFESEQ4097514
Proper citation: RRID:Addgene_246707 Copy
Species: Homo sapiens
Genetic Insert: RECK
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304675
Comments: Gene sourced from Incyte cDNA clone LIFESEQ4097514
Proper citation: RRID:Addgene_246706 Copy
Species: Mus musculus
Genetic Insert: Fzd8
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV-9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304675
Comments: Gene synthetised
Proper citation: RRID:Addgene_246709 Copy
Species: E. coli
Genetic Insert: DNA polymerase III subunit Delta'
Vector Backbone Description: Backbone Size:5181; Vector Backbone:pet28PP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38490435
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.30.569468v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_217410 Copy
Genetic Insert: SARS-CoV Spike AA781-800
Vector Backbone Description: Backbone Size:455; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37977224
Comments: Please visit https://doi.org/10.1101/2023.07.27.550841 for bioRxiv preprint.
Proper citation: RRID:Addgene_210861 Copy
Species: E. coli
Genetic Insert: Beta sliding clamp
Vector Backbone Description: Backbone Size:5242; Vector Backbone:pet28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38490435
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.30.569468v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_217412 Copy
Species: Arabidopsis thaliana
Genetic Insert: Suc2 promoter
Vector Backbone Description: Backbone Size:2249; Vector Backbone:pICSL01008; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 promoter module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245662 Copy
Species: Homo sapiens
Genetic Insert: eIF2 alpha
Vector Backbone Description: Vector Backbone:pBIG1x; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41432235
Proper citation: RRID:Addgene_248659 Copy
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pICH47781; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making full transcriptional unit modules in Level 1 position 6 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1092, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245703 Copy
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pICSL47822; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making full transcriptional unit modules in Level 1 position 3 (REVERSE) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1092, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245707 Copy
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pICSL47811; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making full transcriptional unit modules in Level 1 position 2 (REVERSE) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1092, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245706 Copy
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pICH47751; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making low copy full transcriptional unit modules in Level 1 position 3 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 596, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245714 Copy
Species: Synthetic
Genetic Insert: ZmUBI Promoter-scFV-sfGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pGGD000; Vector Types:GreenGate cloning entry vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246802 Copy
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pICH47732; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making low copy full transcriptional unit modules in Level 1 position 1 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 596, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245712 Copy
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