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Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2100; Vector Backbone:pMOD4; Vector Types:Recombineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19116030
Comments: Few mismatches between Addgene and depositor sequence are in the 3' UTR and shouldn't have any impact on plasmid's function
Proper citation: RRID:Addgene_19185 Copy
Species: Homo sapiens
Genetic Insert: Transforming growth factor, beta receptor II
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See author map for cloning details.
Proper citation: RRID:Addgene_19158 Copy
Species: jellyfish
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4823; Vector Backbone:PL-452; Vector Types:Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18676454
Proper citation: RRID:Addgene_19173 Copy
Species: Drosophila melanogaster
Genetic Insert: posterior sex combs
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11583617
Comments: For baculoviral expression of PSC with N-terminal flag epitope. (kingston #1322)
Proper citation: RRID:Addgene_1926 Copy
Species: Mus musculus
Genetic Insert: cFOS
Vector Backbone Description: Backbone Size:3000; Vector Backbone:BSSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_19259 Copy
Species: Homo sapiens
Genetic Insert: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003620
Comments: human BRG1 cDNA from pFASTBAC-BRG1 (Kingston's lab). Please note this contains an S746L mutation, which has no functional consequence.
Proper citation: RRID:Addgene_19143 Copy
Species: Homo sapiens
Genetic Insert: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003620
Comments: human BRGI with Flag at C-terminus PCR product cut with SalI then blunted and ligated into blunt BamHI site in vector
Proper citation: RRID:Addgene_19148 Copy
Species: Homo sapiens
Genetic Insert: Transforming growth factor, beta receptor II
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003620
Proper citation: RRID:Addgene_19147 Copy
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2348; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19364 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17220275
Proper citation: RRID:Addgene_19365 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting region
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
Proper citation: RRID:Addgene_19330 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pFA6a-5FLAG-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19342 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P41nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19349 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Note from the depositor: This plasmid does not express well. This is because it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells.
Proper citation: RRID:Addgene_19314 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Proper citation: RRID:Addgene_19317 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
Proper citation: RRID:Addgene_19316 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19319 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5568; Vector Backbone:pGL2Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10602481
Comments: Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)
33 bp linker between Ecad promoter and luciferace gene.
Proper citation: RRID:Addgene_19290 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5568; Vector Backbone:pGL2Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11912130
Comments: Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)
33 bp linker between Ecad promoter and luciferace gene.
Proper citation: RRID:Addgene_19291 Copy
Species: Mus musculus
Genetic Insert: NOS2 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5598; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7692452
Proper citation: RRID:Addgene_19296 Copy
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