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Vector Backbone Description: Backbone Size:5443; Vector Backbone:pICH47791; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Level 1 Acceptor for making low copy full transcriptional unit modules in Level 1 position 7 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 596, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).
Proper citation: RRID:Addgene_245718 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247945 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247946 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247948 Copy
Species: Synthetic
Genetic Insert: GFSTF
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247949 Copy
Species: Synthetic
Genetic Insert: GFSTF
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247951 Copy
Vector Backbone Description: Vector Backbone:pRS835; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41428728
Proper citation: RRID:Addgene_247640 Copy
Species: P. patens
Genetic Insert: gRNAscaffold-tRNA
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Golden Gate fragments are amplified by PCR, with primers designed to produce half of each gRNA followed by BsaI recognition sites. Complete gRNA sequences to be reconstituted upon BsaI digestion and ligation during the Golgen Gate reaction.
Proper citation: RRID:Addgene_248333 Copy
Vector Backbone Description: Vector Backbone:pRS1003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41428728
Proper citation: RRID:Addgene_247641 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40233945
Proper citation: RRID:Addgene_206339 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41453699
Proper citation: RRID:Addgene_206338 Copy
Genetic Insert: Lifeact
Vector Backbone Description: Vector Backbone:pENTR2b; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41495248
Comments: First, gene blocks containing the lifeact sequence (Riedl et al., 2008) in frame with mNeonGreen (Shaner et al., 2013) and flanked with Kpn1 and Xho1 restriction sites were purchased from IDT (Integrated DNA Technologies, Inc.). Through enzymatic digestion (Kpn1, ThermoFisher Scientific, FD0524, and XhoI, ThermoFisher Scientific, FD0694) of the two sites on both gene blocks and the pENTR2b entry vector (ThermoFisher Scientific, A10463), we obtained pENTR2b-Lifeact-mNeonGreen.
Please visit https://doi.org/10.1101/2024.09.30.615654 for the bioRxiv preprint.
Proper citation: RRID:Addgene_225496 Copy
Species: Homo sapiens
Genetic Insert: Kappa Opioid Receptor
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39920410
Proper citation: RRID:Addgene_231811 Copy
Genetic Insert: Lifeact
Vector Backbone Description: Vector Backbone:pLenti6.3/TO/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41495248
Comments: Please visit https://doi.org/10.1101/2024.09.30.615654 for the bioRxiv preprint.
Proper citation: RRID:Addgene_225495 Copy
Species: Homo sapiens
Genetic Insert: eIF2 beta
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41432235
Proper citation: RRID:Addgene_248692 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41453699
Proper citation: RRID:Addgene_206333 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40233945
Proper citation: RRID:Addgene_206332 Copy
Species: Mus musculus
Genetic Insert: Adcy3
Vector Backbone Description: Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40472089
Proper citation: RRID:Addgene_245988 Copy
Genetic Insert: puromycin resistant marker
Vector Backbone Description: Backbone Marker:our team; Vector Backbone:pDNR-mCherry-T2A-3xPuroR-Frame 0; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_245866 Copy
Species: Staphylococcus aureus
Genetic Insert: VraS
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41453699
Proper citation: RRID:Addgene_206335 Copy
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