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Species: Mus musculus
Genetic Insert: kif13b
Vector Backbone Description: Backbone Marker:thermo fisher (Invitrogen); Backbone Size:4810; Vector Backbone:pFastBac HTa; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33443153
Proper citation: RRID:Addgene_163908 Copy
Species: Mus musculus
Genetic Insert: kif13b
Vector Backbone Description: Backbone Marker:thermo fisher (Invitrogen); Backbone Size:4812; Vector Backbone:pFastBac HTa; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33443153
Proper citation: RRID:Addgene_163907 Copy
Species: Mus musculus
Genetic Insert: Mau2
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163881 Copy
Species: Mus musculus
Genetic Insert: Mau2
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163880 Copy
Species: Mus musculus
Genetic Insert: Nipbl
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163883 Copy
Species: Mus musculus
Genetic Insert: Nipbl
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163882 Copy
Species: Mus musculus
Genetic Insert: Rbp1
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163884 Copy
Species: Mus musculus
Genetic Insert: PKC delta isoform 2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16389 Copy
Species: Mus musculus
Genetic Insert: PKC delta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16388 Copy
Species: Mus musculus
Genetic Insert: PKC delta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16387 Copy
Species: Mus musculus
Genetic Insert: PKC delta isoform 2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16386 Copy
Species: Mus musculus
Genetic Insert: PKC beta II
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16385 Copy
Species: Mus musculus
Genetic Insert: Ctcf
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163870 Copy
Species: Mus musculus
Genetic Insert: Spt5
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163872 Copy
Species: Mus musculus
Genetic Insert: Rad21
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163871 Copy
Species: Mus musculus
Genetic Insert: Wapl
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163873 Copy
Species: Mus musculus
Genetic Insert: Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26550827
Comments: For pUASTLOTattB_VHH-GFP4::CD8::mCherry, we inserted the VHH-GFP4 fragment after the signal peptide sequence of the mouse CD8 domain in the pUAS::CD8::GFP plasmid. We replaced the GFP by a mCherry (Clonetech) and finally cloned the VHH-GFP4::CD8::mCherry fragment into the pUASTLOTattB vector.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCD8 plasmid:
Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of
gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999).
mCherry: Clonetech
Proper citation: RRID:Addgene_163917 Copy
Species: Mus musculus
Genetic Insert: Brm
Vector Backbone Description: Vector Backbone:pflox; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824
Proper citation: RRID:Addgene_163876 Copy
Species: Mus musculus
Genetic Insert: Partial sense strand of the mouse Tbxas1 gene for in situ hybridization
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4000; Vector Backbone:pCR-BluntII-TOPO; Vector Types:For IVF; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170307 Copy
Species: Mus musculus
Genetic Insert: Partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4000; Vector Backbone:pCR-BluntII-TOPO; Vector Types:For IVF; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170308 Copy
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