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Species: Homo sapiens
Genetic Insert: SHIP1deltaC2
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6067; Vector Backbone:pKan S97Split; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35603158
Proper citation: RRID:Addgene_183769 Copy
Species: Other
Genetic Insert: BFP
Vector Backbone Description: Backbone Size:3125; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27729526
Proper citation: RRID:Addgene_86457 Copy
Species: Homo sapiens
Genetic Insert: SLC35A1
Vector Backbone Description: Vector Backbone:pEGFP.N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34384782
Proper citation: RRID:Addgene_186281 Copy
Species: Homo sapiens
Genetic Insert: high mobility group box 1
Vector Backbone Description: Backbone Marker:system biosciences; Backbone Size:7367; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097772
Proper citation: RRID:Addgene_172357 Copy
Vector Backbone Description: Backbone Size:11415; Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022168
Proper citation: RRID:Addgene_185474 Copy
Species: Homo sapiens
Genetic Insert: KIF1C
Vector Backbone Description: Vector Backbone:pFastbacM13; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35961316
Proper citation: RRID:Addgene_185978 Copy
Species: Homo sapiens
Genetic Insert: KIF1C
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35961316
Proper citation: RRID:Addgene_185981 Copy
Species: Synthetic
Genetic Insert: er-(n2)oxStayGold(c4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35468954
Proper citation: RRID:Addgene_185822 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV116; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177700 Copy
Species: Danio rerio
Genetic Insert: npas4l
Vector Backbone Description: Backbone Size:4063; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044573
Comments: Addgene NGS result had deletion of amino acids 349-352 (TSSS). Depositor confirmed that this deletion does not affect the plasmid function.
Proper citation: RRID:Addgene_164654 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pcS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33912378
Proper citation: RRID:Addgene_178425 Copy
Species: Synthetic
Genetic Insert: er-(n2)oxStayGold(c4)v2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35468954
Proper citation: RRID:Addgene_186296 Copy
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_185481 Copy
Species: Synthetic
Genetic Insert: cmv-mCherry-10xPP7
Vector Backbone Description: Backbone Size:4718; Vector Backbone:pKAN; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185796 Copy
Species: Synthetic
Genetic Insert: cmv-mCherry-8xPP7
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pKAN; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185795 Copy
Species: Synthetic
Genetic Insert: sfGFP-GB1-scAB
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185794 Copy
Species: Synthetic
Genetic Insert: pcp-12xSunTag
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185799 Copy
Species: E. coli
Genetic Insert: 1-phosphofructokinase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10833389
Proper citation: RRID:Addgene_186256 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
Vector Backbone Description: Backbone Size:6311; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35990749
Comments: This plasmid is based on the pUCmini-iCAP-PHP.eB plasmid. Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_184592 Copy
Species: Mus musculus
Genetic Insert: Caspase-11 (AA100-373)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34734155
Proper citation: RRID:Addgene_183382 Copy
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