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Species: Feline Immunodeficiency Virus
Genetic Insert: Gag-Pol and Rev
Vector Backbone Description: Backbone Size:12905; Vector Backbone:modified pUC119; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
Proper citation: RRID:Addgene_1732 Copy
Vector Backbone Description: Backbone Marker:IDT; Vector Backbone:pUCIDT-KAN; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35259873
Proper citation: RRID:Addgene_173224 Copy
Species: Synthetic
Genetic Insert: MCP-mCherry-GFP11-GB1
Vector Backbone Description: Backbone Size:6589; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173216 Copy
Species: Shewanella oneidensis, Bacillus subtilis
Genetic Insert: SO_4388(REC)-PsdR(DBD) 137
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33972436
Proper citation: RRID:Addgene_173171 Copy
Species: Mus musculus
Genetic Insert: Autophagy Related 7
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152324
Proper citation: RRID:Addgene_173170 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA) to co-target ATP1A1 and RUNX1 to perform coselection for RUNX1 +1 ATG insertion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173213 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS
Vector Backbone Description: Backbone Size:7514; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173178 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173211 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS backbone
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4837; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173175 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:6479; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173173 Copy
Species: Danio rerio
Genetic Insert: kcnj2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173127 Copy
Species: Danio rerio
Genetic Insert: kcnj1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173126 Copy
Species: Synthetic
Genetic Insert: pmCDA1-T7RNAP
Vector Backbone Description: Backbone Size:5340; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33406230
Proper citation: RRID:Addgene_173124 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17316 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17317 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17320 Copy
Species: Mus musculus
Genetic Insert: MED10
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins.
Proper citation: RRID:Addgene_17321 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17324 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7499314
Proper citation: RRID:Addgene_17327 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173199 Copy
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