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Species: Homo sapiens
Genetic Insert: Human nucleic acid-binding protein 1 (hNABP1)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550690
Proper citation: RRID:Addgene_128306 Copy
Species: P. aeruginosa
Genetic Insert: Exotoxin A
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128397 Copy
Species: C. diphtheriae
Genetic Insert: Diphtheria toxin
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128395 Copy
Species: Homo sapiens
Genetic Insert: chimeric protein hNABP2N+hNABP1C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550690
Comments: Y85C mutation does not affect plasmid function. Point mutation is located in the middle of the OB fold, and no particular function is reported/known for this residue.
Proper citation: RRID:Addgene_128313 Copy
Species: E. coli
Genetic Insert: Maltose binding protein
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Comments: Depositor confirms mutation does not affect plasmid function.
Proper citation: RRID:Addgene_128390 Copy
Species: N. meningitidis
Genetic Insert: PorA
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128392 Copy
Species: H. influenzae
Genetic Insert: Protein D
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128391 Copy
https://dgrc.bio.indiana.edu/stock/1660656
Species: Drosophila melanogaster
Genetic Insert: ss
Comments: Tagged-Tf BACs
Proper citation: RRID:DGRC_1660656 Copy
https://dgrc.bio.indiana.edu/stock/1660664
Species: Drosophila melanogaster
Genetic Insert: toe
Comments: Tagged-Tf BACs
Proper citation: RRID:DGRC_1660664 Copy
https://dgrc.bio.indiana.edu/stock/1623226
Species: Drosophila melanogaster
Genetic Insert: Ir48c
Comments: BDGP Gold cDNAs
Proper citation: RRID:DGRC_1623226 Copy
https://dgrc.bio.indiana.edu/stock/1647367
Species: Drosophila melanogaster
Genetic Insert: Rdh
Comments: BDGP Gold cDNAs
Proper citation: RRID:DGRC_1647367 Copy
https://dgrc.bio.indiana.edu/stock/1660788
Species: Drosophila melanogaster
Genetic Insert: her
Comments: Tagged-Tf BACs
Proper citation: RRID:DGRC_1660788 Copy
https://dgrc.bio.indiana.edu/stock/1660806
Species: Drosophila melanogaster
Genetic Insert: Sox14
Comments: Tagged-Tf BACs
Proper citation: RRID:DGRC_1660806 Copy
Species: Synthetic
Genetic Insert: MS2-NLS, codon-optimised for Arabidopsis
Vector Backbone Description: Backbone Size:2149; Vector Backbone:pMK-RQ; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 380-385p
MS2-NLS coding sequence = 393-872bp
BsaI site #2 = 878-883bp
Proper citation: RRID:Addgene_122837 Copy
Species: Homo sapiens
Genetic Insert: SHP-2CS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3500; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15133037
Proper citation: RRID:Addgene_12284 Copy
Species: Homo sapiens
Genetic Insert: tUI
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122661 Copy
Species: Homo sapiens
Genetic Insert: tISR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122664 Copy
Species: Homo sapiens
Genetic Insert: tIVR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122663 Copy
Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_122652 Copy
Species: Listeria innocua
Genetic Insert: DPS, listeria
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14514305
Comments: This plasmid is also used in the following publications:
Resnick, D. A., Gilmore, K., Idzerda, Y. U., Klem, M. T., Allen, M., Douglas, T., … Young, M. (2006). Magnetic properties of Co 3O 4 nanoparticles mineralized in Listeria innocua Dps. Journal of Applied Physics, 99(8), 1–4. https://doi.org/10.1063/1.2163839
Okuda, M., Suzumoto, Y., Iwahori, K., Kang, S., Uchida, M., Douglas, T., & Yamashita, I. (2010). Bio-templated CdSe nanoparticle synthesis in a cage shaped protein, Listeria-Dps, and their two dimensional ordered array self-assembly. Chemical Communications, 46(46), 8797–8799. https://doi.org/10.1039/c0cc03298k
Usselman, R. J., Russek, S. E., Klem, M. T., Allen, M. A., Douglas, T., Young, M., … Singel, D. J. (2012). Temperature dependence of electron magnetic resonance spectra of iron oxide nanoparticles mineralized in Listeria innocua protein cages. Journal of Applied Physics, 112(8), 1–9. https://doi.org/10.1063/1.4757964
Proper citation: RRID:Addgene_122654 Copy
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