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Species: Mus musculus
Genetic Insert: CShroom3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2597; Vector Backbone:pDONR221 p5-p2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36104355
Comments: Please visit https://doi.org/10.1101/2021.04.20.440475 for bioRxiv preprint.
Proper citation: RRID:Addgene_170977 Copy
Species: Mus musculus
Genetic Insert: CA-ChREBP
Vector Backbone Description: Vector Backbone:pMSCVhyg-3xT7; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33888555
Proper citation: RRID:Addgene_171029 Copy
Species: Mus musculus
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_171102 Copy
Species: Mus musculus
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_171100 Copy
Species: Mus musculus
Genetic Insert: MAP4-MTBD
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Comments: This is the Microtubule binding domain of MAP4.
Proper citation: RRID:Addgene_171487 Copy
Species: Mus musculus
Genetic Insert: Insulator BRE-MLP:H2B-Turqoise
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pGEM47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33999917
Comments: Addgene QC NGS analysis was unable to generate a full plasmid sequence. The depositing laboratory has indicated that these sequence discrepancies should not be of any consequence to function. Please visit https://www.biorxiv.org/content/10.1101/2020.07.12.198994v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_171499 Copy
Species: Mus musculus
Genetic Insert: MAP4-MTBD
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31249032
Comments: Microtubule binding domain of mouse MAP4 (amino acids 658-1126).
Proper citation: RRID:Addgene_171498 Copy
Species: Mus musculus
Genetic Insert: ATP10B
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32172343
Proper citation: RRID:Addgene_171791 Copy
Species: Mus musculus
Genetic Insert: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33972728
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
Proper citation: RRID:Addgene_171693 Copy
Species: Mus musculus
Genetic Insert: ATP13A2
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31996848
Proper citation: RRID:Addgene_171771 Copy
Species: Mus musculus
Genetic Insert: Bin1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32170110
Proper citation: RRID:Addgene_168280 Copy
Species: Mus musculus
Genetic Insert: hU6-sgBmal1-1-hU6-sgBmal1-3
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: When comparing to the depositor reference sequence, please note that the mutations K14E, N210D, and E249G in mCherry-KASH do not alter plasmid function.
Proper citation: RRID:Addgene_168296 Copy
Species: Mus musculus
Genetic Insert: hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: When comparing to the depositor reference sequence, please note that the mutations K14E, N210D, and E249G in mCherry-KASH do not alter plasmid function.
Proper citation: RRID:Addgene_168281 Copy
Species: Mus musculus
Genetic Insert: Bin1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32170110
Proper citation: RRID:Addgene_168283 Copy
Species: Mus musculus
Genetic Insert: dnWnt-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8946920
Comments: Plasmid History:
Constructed by Jeff Brown on date of 8/8/95.
Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.
PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD. The Wnt-1 gene is truncated after the SPNFC peptide sequence.
Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.
Proper citation: RRID:Addgene_16860 Copy
Species: Mus musculus
Genetic Insert: Synaptophysin-pHluorin
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27429220
Proper citation: RRID:Addgene_168510 Copy
Species: Mus musculus
Genetic Insert: dnWnt-1 myc CS2+
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Transcription: For sense, linearize with Asp718 (BMB) and transcribe with.
SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also.
referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene.
is truncated acter the SPNFC peptide sequence.
Notes for Use:
Constructed by J. Brown.
Proper citation: RRID:Addgene_16859 Copy
Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16854 Copy
Species: Mus musculus
Genetic Insert: antisense Wnt-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Antisense orientation of full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16852 Copy
Species: Mus musculus
Genetic Insert: Wnt-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Full coding sequence of mouse Wnt-1, untagged. ~1.5kb insert isolated from Ajamete Kaykas's 2294 mWnt1 plasmid, Not1filled/Spe1 fragment cloned into pCS2P+ Stu1/Xba1.
Notes for Use: For in vitro transcription linearize with Asp718 and transcribe with SP6.
Reference: Clone made by Gilbert Weidinger, Moon lab.
Proper citation: RRID:Addgene_16850 Copy
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