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Vector Backbone Description: Backbone Marker:Takara; Backbone Size:9700; Vector Backbone:pGADT7 (Gateway adapted); Vector Types:Yeast Expression, Yeast 2-hybrid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17911168
Comments: This plasmid originated from John Doonan's laboratory and is provided, with permission, as an experimental control for the pDEST-GADT7-RGS19 plasmid that is described as a teaching resource. For all other uses please cite PMID: 17911168.
Proper citation: RRID:Addgene_248657 Copy
Species: Synthetic
Genetic Insert: pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
Vector Backbone Description: Vector Backbone:enIscB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247331 Copy
Species: Synthetic
Genetic Insert: pCAG_enDelIscB(D60A)-2×TadA8e(V106W)_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
Vector Backbone Description: Vector Backbone:enIscB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247332 Copy
Species: Mus musculus
Genetic Insert: Cyri-A
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34165494
Comments: Laura Machesky's laboratory is now in the Department of Biochemistry at the University of Cambridge
Proper citation: RRID:Addgene_247175 Copy
Species: Synthetic
Genetic Insert: pCAG_enDelIscB-T5E_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
Vector Backbone Description: Vector Backbone:enIscB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247330 Copy
Species: Mus musculus
Genetic Insert: cpSFGFP.mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38748581
Proper citation: RRID:Addgene_248075 Copy
Genetic Insert: Poetur hyPB
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41039042
Proper citation: RRID:Addgene_248198 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Wilson; Backbone Size:5042; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24267638
Proper citation: RRID:Addgene_247941 Copy
Species: Synthetic
Genetic Insert: PBfP2E5-RBS1PLP-PhlF-rrnBT1-T7Te
Vector Backbone Description: Vector Backbone:pMB1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248762 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247393 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247396 Copy
Species: E. coli
Genetic Insert: MSP1D1_sfCherry
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248923 Copy
Species: E. coli
Genetic Insert: MSP1D1_sfCherryL
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248924 Copy
Species: Synthetic
Genetic Insert: T7Te-rrnBT1-40xTetO
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248767 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0722-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248730 Copy
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Intermediate vector, referred to as a gRNA insert vector, is used to clone guide RNA sequences by annealing complementary oligonucleotides with appropriate overhangs and inserting them into a BbsI site positioned between the PpU6 promoter and the gRNA scaffold. The PpU6 promoter and gRNA scaffold are flanked by outward-facing BsaI sites with unique four-base overhangs, enabling directional and ordered assembly via Golden Gate.
Proper citation: RRID:Addgene_248335 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0733-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248731 Copy
Species: Homo sapiens
Genetic Insert: Arl13B
Vector Backbone Description: Backbone Marker:Lu Lab (Madugula et al J Cell Sci. 2016); Backbone Size:5406; Vector Backbone:pET30ax; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36907439
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_247240 Copy
Species: Homo sapiens
Genetic Insert: eIF2 gamma
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41432235
Proper citation: RRID:Addgene_248693 Copy
Species: Synthetic
Genetic Insert: PBfP2E5-RBS1PLP-LacI-rrnBT1-pLacI43-RBS1PLP-sfGFP-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248736 Copy
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