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Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-2 (ZF 42-10) are upstream of the promoter and ZF-1 (ZF 43-8) binding sites are downstream.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213837 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) and ZF-2 (ZF 42-10) are upstream of the promoter.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213834 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) and ZF-2 (ZF 42-10) are upstream of the promoter.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213836 Copy
Species: Other
Genetic Insert: pSu9 (Presequence of Fo-ATPase subunit 9)
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215001 Copy
Species: Homo sapiens
Genetic Insert: LGALS3
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215003 Copy
Species: Synthetic
Genetic Insert: SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215252 Copy
Species: Synthetic
Genetic Insert: SF-MAR10-SF-nptII-CPH-LUZ-H3H-MAR10-SF
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215256 Copy
Species: Homo sapiens
Genetic Insert: Rab11A
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Gateway DONR; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_228033 Copy
Species: Homo sapiens
Genetic Insert: CASP1[CARD]-mSG2
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway DONR; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_228043 Copy
Species: Synthetic
Genetic Insert: promoter MinSyn104
Vector Backbone Description: Backbone Size:2251; Vector Backbone:Level 0 - promoter -like; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36499768
Proper citation: RRID:Addgene_219725 Copy
Species: Methanomethylophilus alvus
Genetic Insert: M. alvus PylRS 3-NY/HaloY F5
Vector Backbone Description: Backbone Size:3219; Vector Backbone:pAJE-Ma; Vector Types:Methanomethylophilus alvus; Bacterial Resistance:Spectinomycin
Comments: Expresses a) Methanomethylophilus alvus pyrrolysine tRNA synthetase (MaPylRS) engineered for 3-nitrotyrosine and 3-halotryosines under GlnS promoter and b) Methanomethylophilus alvus pyrrolysine tRNA under an Lpp promoter(6). Used as a control for Ma Pyl tRNA-synthetase validation for BL21 or similar cell lines. This plasmid expresses the MaRS and Pyl tRNA and must be paired with a plasmid containing a gene of interest with an amber codon for nitroTyr/haloTyr incorporation.
Proper citation: RRID:Addgene_225684 Copy
Vector Backbone Description: Backbone Size:1961; Vector Backbone:pCOLA-Spec-EV; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39059674
Comments: Please see our protocol for more information on using these plasmids: Two-Stage Dynamic Control Protocol for Cloning, Autoinduction, Autolysis, and Purification of Nanobodies from the E. coli Cytoplasm dx.doi.org/10.17504/protocols.io.j8nlk95d1v5r/v1
Proper citation: RRID:Addgene_212095 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sulfydryl oxidase
Vector Backbone Description: Backbone Size:1900; Vector Backbone:pCOLA-Spec-EV (Addgene #212095); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39059674
Comments: Please see our protocol for more information on using these plasmids: Two-Stage Dynamic Control Protocol for Cloning, Autoinduction, Autolysis, and Purification of Nanobodies from the E. coli Cytoplasm dx.doi.org/10.17504/protocols.io.j8nlk95d1v5r/v1
Proper citation: RRID:Addgene_212094 Copy
Species: Synthetic
Genetic Insert: Altered epegRNA backbone with cloning sites for gRNA and RTT.
Vector Backbone Description: Vector Backbone:pICH41331; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39242983
Proper citation: RRID:Addgene_227695 Copy
Species: Other
Genetic Insert: Plastid transit peptide fused mCherry-UnaG
Vector Backbone Description: Vector Backbone:pGWB508; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213862 Copy
Species: Other
Genetic Insert: Peroxisome transit peptide fused mCerulean
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213861 Copy
Species: Arabidopsis thaliana
Genetic Insert: chloroplast outer envelope membrane transit peptide fused mVenus
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36398034
Proper citation: RRID:Addgene_213864 Copy
Species: Arabidopsis thaliana
Genetic Insert: RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS)
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37792703
Proper citation: RRID:Addgene_213868 Copy
Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Backbone Size:2221; Vector Backbone:pUdO1s; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38235654
Proper citation: RRID:Addgene_210238 Copy
Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
Vector Backbone Description: Vector Backbone:pK7WG3; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Please note: Plasmid contains a K214N mutation, E660-P692 deletion, and HD701-702NI mutations in the mitoTALE ORF. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_210837 Copy
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