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Species: Synthetic
Genetic Insert: PBfP2E5-RBS1PLP-PhlF-rrnBT1-T7Te-pPhlF133-RBS1PLP-sfGFP-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248739 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT1004-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_248733 Copy
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Intermediate vector, referred to as a gRNA insert vector, is used to clone guide RNA sequences by annealing complementary oligonucleotides with appropriate overhangs and inserting them into a BbsI site positioned between the PpU6 promoter and the gRNA scaffold. The PpU6 promoter and gRNA scaffold are flanked by outward-facing BsaI sites with unique four-base overhangs, enabling directional and ordered assembly via Golden Gate.
Proper citation: RRID:Addgene_248339 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247376 Copy
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Intermediate vector, referred to as a gRNA insert vector, is used to clone guide RNA sequences by annealing complementary oligonucleotides with appropriate overhangs and inserting them into a BbsI site positioned between the PpU6 promoter and the gRNA scaffold. The PpU6 promoter and gRNA scaffold are flanked by outward-facing BsaI sites with unique four-base overhangs, enabling directional and ordered assembly via Golden Gate.
Proper citation: RRID:Addgene_248340 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0779-PhlF-rrnBT2-pPhlF133-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248749 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0779-TetR-rrnBT2-pTetR59-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248743 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247414 Copy
Species: Mus musculus
Genetic Insert: iATPSnFR2.A95K.mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38748581
Proper citation: RRID:Addgene_248071 Copy
Species: Mus musculus
Genetic Insert: iATPSnFR2.A95K.tdTomato
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38748581
Proper citation: RRID:Addgene_248072 Copy
Species: Homo sapiens
Genetic Insert: BCL6
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219031 Copy
Species: Homo sapiens
Genetic Insert: NFIX
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219025 Copy
Species: Homo sapiens
Genetic Insert: PAX5
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219041 Copy
Species: Homo sapiens
Genetic Insert: Cas9/HBB_IVS2/Cas9-TagBFP
Vector Backbone Description: Vector Backbone:pPD008; Vector Types:Mammalian Expression, CRISPR, Cp36 donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41309578
Proper citation: RRID:Addgene_249137 Copy
Species: Homo sapiens
Genetic Insert: Cas9-HBB_IVS2-TagBFP
Vector Backbone Description: Vector Backbone:pPD008; Vector Types:Mammalian Expression, CRISPR, Cp36 donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41309578
Proper citation: RRID:Addgene_249133 Copy
Species: Homo sapiens
Genetic Insert: PSMD14_IVS2-Cas9-TagBFP
Vector Backbone Description: Vector Backbone:pPD008; Vector Types:Mammalian Expression, CRISPR, Cp36 donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41309578
Proper citation: RRID:Addgene_249141 Copy
Species: Homo sapiens
Genetic Insert: EIF4A3_IVS2-Cas9-TagBFP
Vector Backbone Description: Vector Backbone:pPD008; Vector Types:Mammalian Expression, CRISPR, Cp36 donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41309578
Proper citation: RRID:Addgene_249140 Copy
Species: Homo sapiens
Genetic Insert: BAZ1A
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219036 Copy
Species: Homo sapiens
Genetic Insert: RUNX2
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219051 Copy
Species: Homo sapiens
Genetic Insert: mutated super slow human Tyrosinase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_249346 Copy
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