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Species: Homo sapiens
Genetic Insert: Annexin V
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4606; Vector Backbone:pET12a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8781447
Proper citation: RRID:Addgene_19961 Copy
Species: Homo sapiens
Genetic Insert: D2 short variant-monomeric Venus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18668123
Proper citation: RRID:Addgene_19966 Copy
Species: Homo sapiens
Genetic Insert: D2 short variant-C terminal Venus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18668123
Proper citation: RRID:Addgene_19968 Copy
Species: Homo sapiens
Genetic Insert: cullin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Proper citation: RRID:Addgene_19892 Copy
Species: Homo sapiens
Genetic Insert: cullin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5512; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Comments: Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.
Proper citation: RRID:Addgene_19893 Copy
Species: Homo sapiens
Genetic Insert: cullin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Comments: When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.
Proper citation: RRID:Addgene_19896 Copy
Species: Homo sapiens
Genetic Insert: cullin 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Proper citation: RRID:Addgene_19895 Copy
Species: Homo sapiens
Genetic Insert: cyclin E2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9840943
Proper citation: RRID:Addgene_19935 Copy
Species: Homo sapiens
Genetic Insert: CDKN1B cyclin-dependent kinase inhibitor 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9840943
Proper citation: RRID:Addgene_19937 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 K720A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Proper citation: RRID:Addgene_19939 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 Y42A/M43A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Comments: flag tag is a variant: YPYDLPDYA
S2A mutation present but does not appear to affect function
Proper citation: RRID:Addgene_19940 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 delta C22
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Proper citation: RRID:Addgene_19942 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 delta 610-615
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Proper citation: RRID:Addgene_19941 Copy
Species: Homo sapiens
Genetic Insert: NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Comments: N51S in seq does not appear to affect function
Proper citation: RRID:Addgene_19943 Copy
Species: Homo sapiens
Genetic Insert: S-phase kinase-associated protein 2 (p45)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9663463
Proper citation: RRID:Addgene_19946 Copy
Species: Homo sapiens
Genetic Insert: yfpsynhdat
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6516; Vector Backbone:pcihygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12065645
Proper citation: RRID:Addgene_19991 Copy
Species: synthetic gene
Genetic Insert: MSP1E3D1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17213193
Proper citation: RRID:Addgene_20066 Copy
Species: Homo sapiens
Genetic Insert: Neurofibromatosis type I
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:5524; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8628317
Comments: The authors note that there is a mutation in the coding sequence: C3918T
"This created a T to I change within the GAP domain of NF1. However, this
change is outside of the conserved blocks within the GAP domain. This
particular residue (1195 of NF1-GRD shown in figure 4 in the chapter by
M.R. Kim and F. Tamanoi, chapter 6 in Neurofibromatosis type I from
genotype to phenotype, editor by M. Upadhyaya and D. N. Cooper BIOS
scientific publisher) is not conserved among different GAP proteins.
Therefore, we believe that the amino acid change does not affect the GAP
activity. In fact, we have used this construct for the wild-type GAP
activity."
Proper citation: RRID:Addgene_19993 Copy
Genetic Insert: GUS
Vector Backbone Description: Backbone Size:4774; Vector Backbone:pDONR221-P3-P2; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20104 Copy
Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-E2419K
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360675
Proper citation: RRID:Addgene_19994 Copy
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