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Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Please note: Plasmid contains a K214N mutation and Leu insertion after G724 in the mTALEAD ORF, which are not known to affect plasmid function.
Proper citation: RRID:Addgene_210835 Copy
Species: Catharanthus roseus var. LBE
Genetic Insert: T16H2 promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203889 Copy
Species: Catharanthus roseus var. LBE
Genetic Insert: T3O promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203891 Copy
Species: Catharanthus roseus var. LBE
Genetic Insert: T3R promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203892 Copy
Species: Catharanthus roseus var. LBE
Genetic Insert: NMT promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203893 Copy
Species: Caulobacter crescentus
Genetic Insert: DnaN(3' end)-sfGFP
Vector Backbone Description: Vector Backbone:pMCS-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38658616
Proper citation: RRID:Addgene_207246 Copy
Species: Synthetic
Genetic Insert: CrGLK
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pICH41308; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203903 Copy
Species: Synthetic
Genetic Insert: CrGLK (without a stop codon)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pAGM1287; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203904 Copy
Species: Synthetic
Genetic Insert: Stop codon
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Vector Backbone:pAGM1301; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38743349
Proper citation: RRID:Addgene_203905 Copy
Species: Arabidopsis thaliana
Genetic Insert: NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1
Vector Backbone Description: Backbone Marker:Dr. Markéta Šámalová; Backbone Size:18688; Vector Backbone:pOpOn2.1; Vector Types:Plant Expression, Dexamethasone inducible vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39212164
Proper citation: RRID:Addgene_228065 Copy
Species: Arabidopsis thaliana
Genetic Insert: NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3
Vector Backbone Description: Backbone Marker:Dr. Markéta Šámalová; Backbone Size:18688; Vector Backbone:pOpOn2.1; Vector Types:Plant Expression, Dexamethasone inducible vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39212164
Comments: A H162R mutation was found in GUS.
Proper citation: RRID:Addgene_228066 Copy
Species: Homo sapiens
Genetic Insert: S3x6-JunBp
Vector Backbone Description: Backbone Size:2726; Vector Backbone:pDonr-258; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: Entry clone (4-1r): TGF-beta pathway reporter construct using 6 repeats of the mouse homolog of a high affinity Smad3 binding region identified 9kb upstream of the human JUNB promoter (from Ramachandran et al. 2018 https://doi.org/10.7554/elife.31756) linked to a JunB minimal promoter
Proper citation: RRID:Addgene_225668 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1, sfGFP, sacB and eforRED
Vector Backbone Description: Vector Backbone:pVP073; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41415724
Comments: sfGFP and scaffold was amplified from: pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-Bsa1-Scaf-L2.
sacB was amplified from: pEN-R2-SacB-L3.
The base editor expression unit was amplified from: pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1.
eforRed was synthesized.
Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.
Proper citation: RRID:Addgene_222081 Copy
Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/R160a_sgRNA2/R160a
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173756 Copy
Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/R160b_sgRNA2/160b
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173757 Copy
Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/390a_sgRNA2/390a
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173758 Copy
Species: Other
Genetic Insert: TTCG_Stop-tOCS_CGCT
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Backbone Size:2073; Vector Backbone:pAGM9121; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192351 Copy
Species: Arabidopsis thaliana
Genetic Insert: AATG_dttLbCas12a-i_TTCG
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Backbone Size:2073; Vector Backbone:pAGM3946; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192350 Copy
Species: Other
Genetic Insert: Plac-RFP (IPTG-inducible red fluorescent protein)
Vector Backbone Description: Backbone Marker:Chain Biotech; Backbone Size:4759; Vector Backbone:pMTL82341; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36427328
Proper citation: RRID:Addgene_191346 Copy
Species: A. tumefaciens
Genetic Insert: pruR-3xFLAG
Vector Backbone Description: Backbone Size:6761; Vector Backbone:pRA301; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36645284
Comments: PruR is a periplasmic protein from Agrobacterium tumefaciens and can be used in conjunction with pJLG038 or pJLG039, which carry an inducible T7 polymerase. It can be replaced with a gene of interest for high yield protein expression from a native host.
Proper citation: RRID:Addgene_192980 Copy
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