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Species: Homo sapiens
Genetic Insert: SNAIL
Vector Backbone Description: Backbone Marker:Gary Nabel; Backbone Size:7131; Vector Backbone:pPGS-CMV-CITE-Neo(-); Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The pPGS-CMV-CITE-neo vector allows the expression of chimeric transcripts encoding a gene of interest fused to the neomycin resistance gene. Expression of the neomycin resistance protein results from an internal ribosome entry site upstream of the neomycin open reading frame.
Proper citation: RRID:Addgene_25695 Copy
Species: Homo sapiens
Genetic Insert: human IL-17 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5064; Vector Backbone:pGL3-Enhancer; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15459204
Proper citation: RRID:Addgene_25628 Copy
Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:5303; Vector Backbone:pENTR1A-Gent; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16945906
Comments: shRNA expression; miR30-based topology; TRE Pmin promoter + GFP spacer; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE
Proper citation: RRID:Addgene_25749 Copy
Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:4608; Vector Backbone:pENTR1A-Gent; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16945906
Comments: shRNA expression; miR30-based topology; TRE Pmin promoter; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE
Proper citation: RRID:Addgene_25748 Copy
Species: Homo sapiens
Genetic Insert: human IL-17 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5064; Vector Backbone:pGL3-Enhancer; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15459204
Proper citation: RRID:Addgene_25629 Copy
Species: Homo sapiens
Genetic Insert: UBE2J2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2F4W http://www.thesgc.org/structures/2F4W/
Proper citation: RRID:Addgene_25624 Copy
Species: Homo sapiens
Genetic Insert: CAPN8
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2NQA http://www.thesgc.org/structures/2NQA/
Proper citation: RRID:Addgene_25623 Copy
Species: Mus musculus
Genetic Insert: Gb2
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13217; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16945906
Comments: Lentivirus; Inducible TRE driven GFP-shRNA (GB2); Constitutive Ubi-c driven rtTA3-IRES-Neo
GB2 miR-shRNA: TGCTCATGTATTCCCACGACAA
Proper citation: RRID:Addgene_25746 Copy
Species: Mus musculus
Genetic Insert: G alpha 12 miR-shRNA
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12370; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16945906
Comments: pSLIK-Venus with G alpha 12 miR-shRNA
G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT
Proper citation: RRID:Addgene_25740 Copy
Species: Homo sapiens
Genetic Insert: CAPN13
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2I7A http://www.thesgc.org/structures/2I7A/
Proper citation: RRID:Addgene_25622 Copy
Species: Homo sapiens
Genetic Insert: UEV3
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2I6T http://www.thesgc.org/structures/2I6T/
Proper citation: RRID:Addgene_25621 Copy
Species: Mus musculus
Genetic Insert: G alpha 13 and 12 miR-shRNA
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12752; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16945906
Comments: pSLIK-Venus with G alpha 12 and 13 miR-shRNA
G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT
G alpha 13 miR-shRNA: CTGGGTGAGTCTGTAAAGTATT
There are 3 minor changes between depositor's sequence and Addgene's sequence - An extra T between the TRE promoter and first shRNA; a 17nt stretch between the shRNAs is different but will not affect function; and a single nt change mutates an EcoRI site just beyond the 2nd shRNA but will again not affect function.
Proper citation: RRID:Addgene_25742 Copy
Species: Homo sapiens
Genetic Insert: TORC1 (1-634)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14536081
Proper citation: RRID:Addgene_25718 Copy
Species: Mus musculus
Genetic Insert: MCL1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19433446
Proper citation: RRID:Addgene_25717 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Proper citation: RRID:Addgene_25675 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Proper citation: RRID:Addgene_25674 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: #107
Proper citation: RRID:Addgene_25676 Copy
Species: Homo sapiens
Genetic Insert: E74-like factor 3 (ets domain transcription factor, epithelial-specific )
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:5200; Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_25728 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: #828
Proper citation: RRID:Addgene_25689 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: #824
Proper citation: RRID:Addgene_25686 Copy
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