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Species: Synthetic
Genetic Insert: PTetO-SD8-BE2, PRha-sgRNA4
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107898 Copy
Species: Synthetic
Genetic Insert: PTetO-SD8-BE2, PLac-sgRNA1
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107895 Copy
Species: Synthetic
Genetic Insert: PTetO-sd2U-Cas9, PLacO-sgRNA1
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107893 Copy
Species: Synthetic
Genetic Insert: PTetO-sd2-Cas9, PLac-sgRNA1
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107890 Copy
Species: Synthetic
Genetic Insert: PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107900 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108444 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108441 Copy
Species: Arabidopsis thaliana
Genetic Insert: GA2ox2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108567 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108566 Copy
Species: Arabidopsis thaliana
Genetic Insert: GA2ox2 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108564 Copy
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_108681 Copy
Species: Synthetic
Genetic Insert: Atco-Cas9-Pea3ter
Vector Backbone Description: Backbone Marker:Kohchi Lab., Kyoto; Backbone Size:11315; Vector Backbone:pMpGWB103; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_108722 Copy
Species: Synthetic
Genetic Insert: Taz
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29650958
Proper citation: RRID:Addgene_104333 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104438 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC19
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104684 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC15
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104680 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC16
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104681 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC23
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104688 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC22
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104687 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC31
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104696 Copy
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