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Species: Homo sapiens
Genetic Insert: ITCH
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3TUG. http://www.thesgc.org/structures/3TUG/
Proper citation: RRID:Addgene_36197 Copy
Genetic Insert: yECitrine
Vector Backbone Description: Backbone Marker:Yeast Resource Center, University of Washington; Backbone Size:4882; Vector Backbone:pBS7; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Proper citation: RRID:Addgene_36225 Copy
Genetic Insert: yEVenus
Vector Backbone Description: Backbone Marker:Yeast Resource Center, University of Washington; Backbone Size:4882; Vector Backbone:pBS7; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Proper citation: RRID:Addgene_36224 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: Streptoalloteichus hindustanus bleomycin (Sh ble)
Vector Backbone Description: Vector Backbone:pCY 3080-01; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Comments: The minor discrepancies between the Addgene and author's sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_36227 Copy
Species: Homo sapiens
Genetic Insert: HDAC6.DC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Comments: Additional Reference:
Gao etal "Histone Deacetylase 6 Regulates Growth Factor-Induced Actin Remodeling and Endocytosis" MOLECULAR AND CELLULAR BIOLOGY, Dec. 2007, p. 8637–8647
Proper citation: RRID:Addgene_36189 Copy
Genetic Insert: Homodimeric FokI Nuclease
Vector Backbone Description: Vector Backbone:pCP4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124327
Comments: Note that there are some minor discrepancies between the Addgene quality control sequence and the sequence from the depositor. These differences will not affect the function of the plasmid.
Proper citation: RRID:Addgene_36187 Copy
Genetic Insert: FokI homodimeric Nuclease domain
Vector Backbone Description: Vector Backbone:pCP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124327
Proper citation: RRID:Addgene_36186 Copy
Species: Homo sapiens
Genetic Insert: HDAC6..delta Buz
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Proper citation: RRID:Addgene_36190 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Backbone Marker:Yeast Resource Center, University of Washington; Backbone Size:4882; Vector Backbone:pBS7; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Proper citation: RRID:Addgene_36217 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast-enhanced (yE) polylinker
Vector Backbone Description: Backbone Marker:Yeast Resource Center, University of Washington; Backbone Size:4875; Vector Backbone:pBS10; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22473760
Proper citation: RRID:Addgene_36210 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pmTurquoise2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Comments: Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.
Proper citation: RRID:Addgene_36207 Copy
Species: Homo sapiens
Genetic Insert: Golgi targeting sequence
Vector Backbone Description: Vector Backbone:pmTurquoise2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_36205 Copy
Genetic Insert: HEH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36282 Copy
Species: Homo sapiens
Genetic Insert: Sec23A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21045114
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.
Proper citation: RRID:Addgene_36158 Copy
Genetic Insert: HE-6
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36279 Copy
Species: Homo sapiens
Genetic Insert: Sec16A
Vector Backbone Description: Backbone Marker:Clontech, then modified; Vector Backbone:pVenus-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17005010
Proper citation: RRID:Addgene_36157 Copy
Genetic Insert: HE-5
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36278 Copy
Genetic Insert: EH-4
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36273 Copy
Genetic Insert: EH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36272 Copy
Genetic Insert: EH-2
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36271 Copy
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