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Species: Homo sapiens
Genetic Insert: eGFP-TALEN-08-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCACGCTGCC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39373 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-09-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGCCCTTGCTC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.7kb and 5.8kb
Proper citation: RRID:Addgene_39375 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-09-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAGAGGATCCAC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.7kb and 5.8kb
Proper citation: RRID:Addgene_39374 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-12-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGCGAGCTGC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.7kb and 5.8kb
Proper citation: RRID:Addgene_39381 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:J Exp Med (1986) 164:1478; Backbone Size:3000; Vector Backbone:pcEXV-3; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39528 Copy
Species: Homo sapiens
Genetic Insert: HRAS N17
Vector Backbone Description: Backbone Marker:J Exp Med (1986) 164:1478; Backbone Size:3000; Vector Backbone:pcEXV-3; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39527 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39526 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:Addgene plasmid #1767; Vector Backbone:pBABE-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39525 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 E38
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39520 Copy
Species: Homo sapiens
Genetic Insert: ARAP3(R307,8A)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11804589
Proper citation: RRID:Addgene_39486 Copy
Species: Homo sapiens
Genetic Insert: ARAP3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11804589
Proper citation: RRID:Addgene_39484 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 S35
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39517 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 A38
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39519 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 G37
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39518 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Comments: Please note that the p53 insert in this plasmid contains a known P72R variant when compared to GenBank ID NP_000537.3
Proper citation: RRID:Addgene_39479 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 E38
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39514 Copy
Species: Homo sapiens
Genetic Insert: ARID1A
Vector Backbone Description: Vector Backbone:pLenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Proper citation: RRID:Addgene_39478 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21900401
Comments: Please note that the p53 insert in this plasmid contains a known P72R variant when compared to GenBank ID NP_000537.3
Proper citation: RRID:Addgene_39480 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 E38
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39507 Copy
Species: Homo sapiens
Genetic Insert: HRAS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39503 Copy
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