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Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pKS013; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65466 Copy
Genetic Insert: Ntag-mCherry
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65465 Copy
Genetic Insert: MBP-Ntag-mCherry
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65464 Copy
Species: Staphylococcus aureus
Genetic Insert: mammalian codon-optimized SaCas9, and SaCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65770 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BspMI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65769 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65768 Copy
Species: Synthetic
Genetic Insert: gltA2-udhA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65819 Copy
Species: Synthetic
Genetic Insert: udhA guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65818 Copy
Species: Synthetic
Genetic Insert: zwf guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65825 Copy
Species: Synthetic
Genetic Insert: proD guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65820 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: Integrated into the attP site
Proper citation: RRID:Addgene_65954 Copy
Species: Sulfolobus solfataricus P2
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2206; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20607777
Comments: We cloned the PCNA1 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).
Proper citation: RRID:Addgene_66125 Copy
Species: Other
Genetic Insert: STp
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3284902
Comments: Contains the E. coli STp gene probe (157 bp) cloned on PstI linkers into pBR325 Heat stable enterotoxin gene (porcine)
Proper citation: RRID:Addgene_66149 Copy
Species: Escherichia coli
Genetic Insert: rpsA
Vector Backbone Description: Backbone Size:4520; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31832686
Proper citation: RRID:Addgene_83585 Copy
Species: E. coli
Genetic Insert: Gro EL/ES
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23220215
Proper citation: RRID:Addgene_83923 Copy
Species: Synthetic
Genetic Insert: Glyceraldehyde-3-phosphate dehydrogenase promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84961 Copy
Species: Synthetic
Genetic Insert: Alcohol oxidase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84960 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:9077; Vector Backbone:pMMB207c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28257493
Proper citation: RRID:Addgene_84821 Copy
Vector Backbone Description: Vector Backbone:pms008; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27782150
Proper citation: RRID:Addgene_87957 Copy
Vector Backbone Description: Vector Backbone:PMS028; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27782150
Proper citation: RRID:Addgene_87958 Copy
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