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Species: Drosophila melanogaster
Genetic Insert: RRP40
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6072; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65797 Copy
Species: Drosophila melanogaster
Genetic Insert: Ncd kinesin-14 motor protein
Vector Backbone Description: Backbone Size:7814; Vector Backbone:pCaSpeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9182665
Comments: Other references describing Ncd-GFP expression:
Endow SA & Komma DJ 1996 J Cell Sci 109, 2429-2442 - PubMed ID: 8923204
Skold HN, Komma DJ & Endow SA 2005 J Cell Sci 118, 1745-1755 - PubMed ID: 15797926
Proper citation: RRID:Addgene_66082 Copy
Species: Drosophila melanogaster
Genetic Insert: DIS3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6051; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_66592 Copy
Species: Drosophila melanogaster
Genetic Insert: Hrb98
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6064; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_66595 Copy
Species: Drosophila melanogaster
Genetic Insert: CG13188
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To use this plasmid for S2 cell transfection, a short Kozac sequence was added 5' to the ATG of CG13188 : ccGCG-ATG...
Mutation H452N does not affect plasmid function.
Proper citation: RRID:Addgene_66791 Copy
Species: Drosophila melanogaster
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23819996
Proper citation: RRID:Addgene_66918 Copy
Species: Drosophila melanogaster
Genetic Insert: CPD photolyase
Vector Backbone Description: Backbone Marker:Gibco BRL; Backbone Size:6700; Vector Backbone:pFastbac, modified; Vector Types:bacterial vector, insert not expressed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22175817
Comments: Construct is intermediate in making insect cell virus for expression. *Mutation was found during quality control and is not known to confer any functional change.
Proper citation: RRID:Addgene_67285 Copy
Species: Drosophila melanogaster
Genetic Insert: 6/4 photolyase from Drosophila
Vector Backbone Description: Backbone Marker:Gibco BRL; Backbone Size:5000; Vector Backbone:pFastbac; Vector Types:Intermediate for insect expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22175817
Proper citation: RRID:Addgene_67284 Copy
Species: Drosophila melanogaster
Genetic Insert: 57F03 enhancer
Vector Backbone Description: Vector Backbone:pBPLexAGAD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27040496
Comments: Note: The 57F03 promoter was amplified from fly strain CS while the provided promoter sequence is from fly strain w1118. Therefore, there are some base changes between the provided full plasmid sequence and the actual plasmid (as seen in the Addgene quality control sequencing results) in the promoter region. The depositing laboratory is aware of these base changes; they do not affect plasmid fuction.
Proper citation: RRID:Addgene_83256 Copy
Species: Drosophila melanogaster
Genetic Insert: Ir25a enhancer
Vector Backbone Description: Vector Backbone:pBPGAL80w; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27040496
Comments: Note: The Ir25a promoter was amplified from fly strain CS while the provided promoter sequence is from fly strain w1118. Therefore, there are some base changes between the provided full plasmid sequence and the actual plasmid (as seen in the Addgene quality control sequencing results) in the promoter region. The depositing laboratory is aware of these base changes; they do not affect plasmid fuction.
Proper citation: RRID:Addgene_83259 Copy
Species: Drosophila melanogaster
Genetic Insert: white sgRNA-1
Vector Backbone Description: Backbone Size:4575; Vector Backbone:pCFD4d; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27543296
Proper citation: RRID:Addgene_84005 Copy
Species: Drosophila melanogaster
Genetic Insert: STX1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3538; Vector Backbone:pMT/V5-HIS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89310 Copy
Species: Drosophila melanogaster
Genetic Insert: yki
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18256197
Proper citation: RRID:Addgene_85622 Copy
Species: Drosophila melanogaster
Genetic Insert: Dmp53
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5400; Vector Backbone:pAc5.1/V5 HIS A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18492669
Proper citation: RRID:Addgene_85698 Copy
Species: Drosophila melanogaster
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5400; Vector Backbone:pAc5.1/V5 HIS A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18492669
Proper citation: RRID:Addgene_85696 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86390 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ncm enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86385 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86389 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86387 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86388 Copy
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