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Genetic Insert: 3xFLAG
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78383 Copy
Genetic Insert: 3xPK
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78382 Copy
Genetic Insert: 13xMyc
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78385 Copy
Genetic Insert: 3xHA
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_78384 Copy
Species: Synthetic
Genetic Insert: Streptococcus pyogenes guide RNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3979; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23561441
Proper citation: RRID:Addgene_64711 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6KT-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15908923
Comments: The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_64969 Copy
Species: Synthetic
Genetic Insert: SEP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24403083
Comments: Note that the coding sequence for SEP is lacking a start codon.
Proper citation: RRID:Addgene_64944 Copy
Genetic Insert: FRT-KmR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15908923
Comments: GenBank Accession #AY597272
Compared to the Genbank reference sequence AY597272, Addgene's sequencing results found N18I, R145C and D146N mutations in the kanamycin cassette. The effect of these differences is unknown.
Proper citation: RRID:Addgene_64950 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68034 Copy
Species: Mus musculus
Genetic Insert: Plexin-B3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68037 Copy
Species: Mus musculus
Genetic Insert: Plexin-B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68036 Copy
Species: Mus musculus
Genetic Insert: Plexin-B1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68035 Copy
Species: Bacterial origin
Genetic Insert: Kanamycin cassette
Vector Backbone Description: Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is a second generation plasmid that increases the efficiency of sgRNA guide cloning over the original "pLKO.1-puro U6 sgRNA BspMI stuffer" vector.
"pLKO.1-puro U6 sgRNA BfuAI large stuffer" was created to simplify the cloning of the sgRNA guide cassette into pLKO.1-puro U6 sgRNA BspMI stuffer vector originally described in the manuscript “Kearns, NA, etal. Development 2014, 141(1):219-23”. The original vector has a small intervening sequence that presents two problems: 1) the efficiency of excision by BfuAI is moderate, and 2) the size of this spacer is similar to the guide sequence, which makes distinguishing positive clones from reclosures challenging. Consequently, we have inserted a Kanamycin cassette between these sites at a unique Bcl1 site. This element is more efficiently excised by BfuAI and makes distinguishing vectors containing the desired guide sequence easy to distinguish from the parent vector. The excision of the Kan cassette by BfuAI cuts this cassette into 3 fragments due to two internal BfuAI sites within the Kan cassette. Otherwise the cloning of the guides is carried out as described in the manuscript.
Proper citation: RRID:Addgene_52628 Copy
Genetic Insert: GFP4m
Vector Backbone Description: Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24304893
Proper citation: RRID:Addgene_51545 Copy
Species: Mus musculus
Genetic Insert: mouse Ins2 gene partial
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23144715
Proper citation: RRID:Addgene_53969 Copy
Species: Homo sapiens
Genetic Insert: human INS gene partial
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23144715
Proper citation: RRID:Addgene_53970 Copy
Species: Danio rerio
Genetic Insert: mogat3b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39510175
Proper citation: RRID:Addgene_232136 Copy
Species: Danio rerio
Genetic Insert: dgat2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39510175
Proper citation: RRID:Addgene_232134 Copy
Species: Danio rerio
Genetic Insert: dgat1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39510175
Proper citation: RRID:Addgene_232137 Copy
Vector Backbone Description: Vector Backbone:lvl2 [backbone]; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25378321
Comments: This plasmid is slightly modified from the version used in Duportet et al. 2014, but does not have any functional differences.
Proper citation: RRID:Addgene_229208 Copy
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