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  • Bacterial Resistance:ampicillin and kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pNX3a2-FL3
 
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RRID:Addgene_78383 3xFLAG Ampicillin and Kanamycin PMID:27825338 Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:20:23 0
pNX3a2-PK3
 
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RRID:Addgene_78382 3xPK Ampicillin and Kanamycin PMID:27825338 Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:20:23 0
pNX3a2-Myc13
 
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RRID:Addgene_78385 13xMyc Ampicillin and Kanamycin PMID:27825338 Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:20:23 0
pNX3a2-HA3
 
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RRID:Addgene_78384 3xHA Ampicillin and Kanamycin PMID:27825338 Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:20:23 0
pGuide
 
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10+ mentions
RRID:Addgene_64711 Streptococcus pyogenes guide RNA Synthetic Ampicillin and Kanamycin PMID:23561441 Backbone Marker:Invitrogen; Backbone Size:3979; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:12 15
pUC18R6KT-mini-Tn7T-Km
 
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1+ mentions
RRID:Addgene_64969 Ampicillin and Kanamycin PMID:15908923 The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6KT-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:14 5
SEPTOPO
 
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RRID:Addgene_64944 SEP Synthetic Ampicillin and Kanamycin PMID:24403083 Note that the coding sequence for SEP is lacking a start codon. Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:15 0
pFKM1
 
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RRID:Addgene_64950 FRT-KmR-FRT Ampicillin and Kanamycin PMID:15908923 GenBank Accession #AY597272 Compared to the Genbank reference sequence AY597272, Addgene's sequencing results found N18I, R145C and D146N mutations in the kanamycin cassette. The effect of these differences is unknown. Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:14 0
pCRII-Sema4g-ISH-2kb
 
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RRID:Addgene_68034 Semaphorin-4G Mus musculus Ampicillin and Kanamycin PMID:21122816 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:40 0
pCRII-Plxnb3-ISH-2kb
 
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RRID:Addgene_68037 Plexin-B3 Mus musculus Ampicillin and Kanamycin PMID:21122816 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:40 0
pCRII-Plxnb2-ISH-2kb
 
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1+ mentions
RRID:Addgene_68036 Plexin-B2 Mus musculus Ampicillin and Kanamycin PMID:21122816 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:44 1
pCRII-Plxnb1-ISH-2kb
 
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1+ mentions
RRID:Addgene_68035 Plexin-B1 Mus musculus Ampicillin and Kanamycin PMID:21122816 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:18:40 1
pLKO.1-puro U6 sgRNA BfuAI large stuffer
 
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10+ mentions
RRID:Addgene_52628 Kanamycin cassette Bacterial origin Ampicillin and Kanamycin This is a second generation plasmid that increases the efficiency of sgRNA guide cloning over the original "pLKO.1-puro U6 sgRNA BspMI stuffer" vector. "pLKO.1-puro U6 sgRNA BfuAI large stuffer" was created to simplify the cloning of the sgRNA guide cassette into pLKO.1-puro U6 sgRNA BspMI stuffer vector originally described in the manuscript “Kearns, NA, etal. Development 2014, 141(1):219-23”. The original vector has a small intervening sequence that presents two problems: 1) the efficiency of excision by BfuAI is moderate, and 2) the size of this spacer is similar to the guide sequence, which makes distinguishing positive clones from reclosures challenging. Consequently, we have inserted a Kanamycin cassette between these sites at a unique Bcl1 site. This element is more efficiently excised by BfuAI and makes distinguishing vectors containing the desired guide sequence easy to distinguish from the parent vector. The excision of the Kan cassette by BfuAI cuts this cassette into 3 fragments due to two internal BfuAI sites within the Kan cassette. Otherwise the cloning of the guides is carried out as described in the manuscript. Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:24 23
p2attNG-H11-long
 
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1+ mentions
RRID:Addgene_51545 GFP4m Ampicillin and Kanamycin PMID:24304893 Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:14 1
pCR2.1-Ins2-842
 
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RRID:Addgene_53969 mouse Ins2 gene partial Mus musculus Ampicillin and Kanamycin PMID:23144715 Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:35 0
pCR2.1-INS-904
 
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RRID:Addgene_53970 human INS gene partial Homo sapiens Ampicillin and Kanamycin PMID:23144715 Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:35 0
pCRII-mogat3b in situ probe
 
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RRID:Addgene_232136 mogat3b Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pCRII-dgat2 in situ probe
 
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RRID:Addgene_232134 dgat2 Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pCRII-TOPO-dgat1a in situ probe
 
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RRID:Addgene_232137 dgat1a Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
lvl2-[lenti-backbone]
 
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RRID:Addgene_229208 Ampicillin and Kanamycin PMID:25378321 This plasmid is slightly modified from the version used in Duportet et al. 2014, but does not have any functional differences. Vector Backbone:lvl2 [backbone]; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:46 0

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