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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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pNX3a2-FL3 Resource Report Resource Website |
RRID:Addgene_78383 | 3xFLAG | Ampicillin and Kanamycin | PMID:27825338 | Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:20:23 | 0 | |||
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pNX3a2-PK3 Resource Report Resource Website |
RRID:Addgene_78382 | 3xPK | Ampicillin and Kanamycin | PMID:27825338 | Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:20:23 | 0 | |||
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pNX3a2-Myc13 Resource Report Resource Website |
RRID:Addgene_78385 | 13xMyc | Ampicillin and Kanamycin | PMID:27825338 | Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:20:23 | 0 | |||
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pNX3a2-HA3 Resource Report Resource Website |
RRID:Addgene_78384 | 3xHA | Ampicillin and Kanamycin | PMID:27825338 | Vector Backbone:pFA6a-kanMX6; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:20:23 | 0 | |||
|
pGuide Resource Report Resource Website 10+ mentions |
RRID:Addgene_64711 | Streptococcus pyogenes guide RNA | Synthetic | Ampicillin and Kanamycin | PMID:23561441 | Backbone Marker:Invitrogen; Backbone Size:3979; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:12 | 15 | ||
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pUC18R6KT-mini-Tn7T-Km Resource Report Resource Website 1+ mentions |
RRID:Addgene_64969 | Ampicillin and Kanamycin | PMID:15908923 | The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6KT-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:14 | 5 | |||
|
SEPTOPO Resource Report Resource Website |
RRID:Addgene_64944 | SEP | Synthetic | Ampicillin and Kanamycin | PMID:24403083 | Note that the coding sequence for SEP is lacking a start codon. | Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:15 | 0 | |
|
pFKM1 Resource Report Resource Website |
RRID:Addgene_64950 | FRT-KmR-FRT | Ampicillin and Kanamycin | PMID:15908923 | GenBank Accession #AY597272 Compared to the Genbank reference sequence AY597272, Addgene's sequencing results found N18I, R145C and D146N mutations in the kanamycin cassette. The effect of these differences is unknown. | Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:14 | 0 | ||
|
pCRII-Sema4g-ISH-2kb Resource Report Resource Website |
RRID:Addgene_68034 | Semaphorin-4G | Mus musculus | Ampicillin and Kanamycin | PMID:21122816 | Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:40 | 0 | ||
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pCRII-Plxnb3-ISH-2kb Resource Report Resource Website |
RRID:Addgene_68037 | Plexin-B3 | Mus musculus | Ampicillin and Kanamycin | PMID:21122816 | Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:40 | 0 | ||
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pCRII-Plxnb2-ISH-2kb Resource Report Resource Website 1+ mentions |
RRID:Addgene_68036 | Plexin-B2 | Mus musculus | Ampicillin and Kanamycin | PMID:21122816 | Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:44 | 1 | ||
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pCRII-Plxnb1-ISH-2kb Resource Report Resource Website 1+ mentions |
RRID:Addgene_68035 | Plexin-B1 | Mus musculus | Ampicillin and Kanamycin | PMID:21122816 | Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:18:40 | 1 | ||
|
pLKO.1-puro U6 sgRNA BfuAI large stuffer Resource Report Resource Website 10+ mentions |
RRID:Addgene_52628 | Kanamycin cassette | Bacterial origin | Ampicillin and Kanamycin | This is a second generation plasmid that increases the efficiency of sgRNA guide cloning over the original "pLKO.1-puro U6 sgRNA BspMI stuffer" vector. "pLKO.1-puro U6 sgRNA BfuAI large stuffer" was created to simplify the cloning of the sgRNA guide cassette into pLKO.1-puro U6 sgRNA BspMI stuffer vector originally described in the manuscript “Kearns, NA, etal. Development 2014, 141(1):219-23”. The original vector has a small intervening sequence that presents two problems: 1) the efficiency of excision by BfuAI is moderate, and 2) the size of this spacer is similar to the guide sequence, which makes distinguishing positive clones from reclosures challenging. Consequently, we have inserted a Kanamycin cassette between these sites at a unique Bcl1 site. This element is more efficiently excised by BfuAI and makes distinguishing vectors containing the desired guide sequence easy to distinguish from the parent vector. The excision of the Kan cassette by BfuAI cuts this cassette into 3 fragments due to two internal BfuAI sites within the Kan cassette. Otherwise the cloning of the guides is carried out as described in the manuscript. | Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:16:24 | 23 | ||
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p2attNG-H11-long Resource Report Resource Website 1+ mentions |
RRID:Addgene_51545 | GFP4m | Ampicillin and Kanamycin | PMID:24304893 | Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:16:14 | 1 | |||
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pCR2.1-Ins2-842 Resource Report Resource Website |
RRID:Addgene_53969 | mouse Ins2 gene partial | Mus musculus | Ampicillin and Kanamycin | PMID:23144715 | Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:16:35 | 0 | ||
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pCR2.1-INS-904 Resource Report Resource Website |
RRID:Addgene_53970 | human INS gene partial | Homo sapiens | Ampicillin and Kanamycin | PMID:23144715 | Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:16:35 | 0 | ||
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pCRII-mogat3b in situ probe Resource Report Resource Website |
RRID:Addgene_232136 | mogat3b | Danio rerio | Ampicillin and Kanamycin | PMID:39510175 | Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:31:47 | 0 | ||
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pCRII-dgat2 in situ probe Resource Report Resource Website |
RRID:Addgene_232134 | dgat2 | Danio rerio | Ampicillin and Kanamycin | PMID:39510175 | Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:31:47 | 0 | ||
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pCRII-TOPO-dgat1a in situ probe Resource Report Resource Website |
RRID:Addgene_232137 | dgat1a | Danio rerio | Ampicillin and Kanamycin | PMID:39510175 | Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:31:47 | 0 | ||
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lvl2-[lenti-backbone] Resource Report Resource Website |
RRID:Addgene_229208 | Ampicillin and Kanamycin | PMID:25378321 | This plasmid is slightly modified from the version used in Duportet et al. 2014, but does not have any functional differences. | Vector Backbone:lvl2 [backbone]; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:31:46 | 0 |
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