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Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: #827
Proper citation: RRID:Addgene_25688 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: Site-directed mutagnesis was performed on pEBB XIAP W310A to change H467 to Ala (CAT to GCT). This mutation destroys the E3 Ub ligase function of XIAP.
#723
Proper citation: RRID:Addgene_25682 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: Site-directed mutagnesis was performed on pEBB XIAP W310A to introduce a stop codon at AA 449 (AAG to TGA).
#723
Proper citation: RRID:Addgene_25681 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: XIAP W310A was cloned into the BamHI/NotI sites of pEBB FLAG
#739
Proper citation: RRID:Addgene_25684 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: XIAP D148A was cloned into BamHI/NotI sites of pEBB FLAG.
#738
Proper citation: RRID:Addgene_25683 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: Site-directed mutagenesis was performed on pEBB XIAP to change H467 to Ala (CAT to GCT). This mutation destroys the E3 Ub ligase function of XIAP.
Proper citation: RRID:Addgene_25680 Copy
Species: Homo sapiens
Genetic Insert: U6 snRNP
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9068795
Comments: The U6+27 cassette was constructed by PCR amplification from 265 nucleotides upstream of the human U6 gene transcription initiation site through the first 27 nucleotides of the transcribed gene.
Proper citation: RRID:Addgene_25709 Copy
Species: Homo sapiens
Genetic Insert: Axin 2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11940574
Comments: Contains AXIN2 sequences from -1078 to +5 relative to the presumed transcription start site.
Proper citation: RRID:Addgene_25701 Copy
Species: Homo sapiens
Genetic Insert: Nanog
Vector Backbone Description: Backbone Size:8377; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19898493
Proper citation: RRID:Addgene_25700 Copy
Genetic Insert: SFFV promoter, eGFP
Vector Backbone Description: Backbone Size:6417; Vector Backbone:LeGO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18362927
Comments: HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de
Proper citation: RRID:Addgene_25917 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20581846
Comments: FRB is a small domain derived from full length mTOR. In brief, rapamycin binds to a protein called FKBP12. Rapamycin bound to FKBP12 inhibits mTOR signaling
Proper citation: RRID:Addgene_25919 Copy
Species: Bacillus subtilis
Genetic Insert: His-tagged soluble ResA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16537372
Proper citation: RRID:Addgene_25914 Copy
Species: Mus musculus
Genetic Insert: Ig germline mu switch region
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6278424
Comments: Includes a 5 kb EcoRI/BamHI fragment with Smu (3 kb deletion relative to the germline) and both 5' and 3' flanking sequences.
Proper citation: RRID:Addgene_25912 Copy
Species: Mus musculus
Genetic Insert: Ig germline gamma-1 switch region alpha
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6292864
Comments: Includes a 5 kb fragment germline, which includes most of the Calpha switch region (Salpha) and part of Calpha.
NB: CH switch (S) regions
Proper citation: RRID:Addgene_25911 Copy
Species: A. victoria
Genetic Insert: EYFP H148Q
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11423120
Proper citation: RRID:Addgene_25873 Copy
Species: A. victoria
Genetic Insert: EYFP H148Q
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11423120
Proper citation: RRID:Addgene_25871 Copy
Vector Backbone Description: Backbone Marker:Ivanova, NB; Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371338
Comments: Empty Vector pFUGW-H1.
The H1 promoter was cloned into the PacI site of FUGW (Addgene Plasmid# 14883). Please see cloning protocol for recommended shRNA subcloning procedure.
Proper citation: RRID:Addgene_25870 Copy
Species: Homo sapiens
Genetic Insert: RapR-FAK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20581846
Proper citation: RRID:Addgene_25928 Copy
Species: Homo sapiens
Genetic Insert: PQBP1 with Y65C mutation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA4-His-MaxA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20410308
Proper citation: RRID:Addgene_25803 Copy
Genetic Insert: alternative hsa-miR-34a promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Comments: promoter region of the alternative pri-miR-34a transcript identified by Navarro et al. Blood 2009 Sep 3;114(10):2181-92.
There are several discrepancies between this sequence and the GenBank sequence: T(at position 388) is cahnged to C
"ACACAC" insertion at position 658
"T" insertion at position 1914
A (at position 1599) changed to G
T (at position 1480) changed to G
G (at position 1212) changed to A
T (at position 1199) changed to C
These changes were present in all the clones that the lab sequenced and these clones came from two independent PCRs using genomic DNA extracted from the human erithroleukemia cell line K562. The changes might be due to that particular cell line. Importantly, all the minipreps I tested induced luciferase activity in K562 cells upon TPA treatment (as reported in the manuscript).
Proper citation: RRID:Addgene_25800 Copy
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