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Genetic Insert: DsRed
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pDONR221-P4R-P3R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20099 Copy
Genetic Insert: GFPGUS
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pDONR221-P4R-P3R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20098 Copy
Species: CaMV
Genetic Insert: 2x35S
Vector Backbone Description: Backbone Size:4777; Vector Backbone:pDONR221-P4P1R; Vector Types:Synthetic Biology, Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20093 Copy
Vector Backbone Description: Backbone Size:4525; Vector Backbone:p1NIL; Vector Types:Suicide vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20187 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FKBP
Proper citation: RRID:Addgene_20220 Copy
Species: Mus musculus
Genetic Insert: Notch 3 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3
(5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
Proper citation: RRID:Addgene_20185 Copy
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pBR322; Vector Types:Marker cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20189 Copy
Species: Mus musculus
Genetic Insert: Notch 2 intracellular domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
(5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3').
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
Proper citation: RRID:Addgene_20184 Copy
Genetic Insert: amorphadiene synthase
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425GAL1; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16612385
Proper citation: RRID:Addgene_20119 Copy
Species: A. annua
Genetic Insert: Amorphadiene oxidase and p450 reductase
Vector Backbone Description: Backbone Marker:P. Ortiz de Montellano, UCSF; Backbone Size:0; Vector Backbone:pCWori; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17438551
Proper citation: RRID:Addgene_20117 Copy
Species: Homo sapiens
Genetic Insert: NRas
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5607; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20205 Copy
Genetic Insert: shp53/GFP4
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5158; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20208 Copy
Species: Drosophila melanogaster
Genetic Insert: spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19029882
Comments: There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function.
Proper citation: RRID:Addgene_20163 Copy
Species: Mus musculus
Genetic Insert: C-Fluc
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:6082; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20203 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9637; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20205919
Comments: Two mutations are present in the LEU2 sequence: D52E and V69A
Proper citation: RRID:Addgene_20161 Copy
Species: Homo sapiens
Genetic Insert: CFP-FKBP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15908919
Proper citation: RRID:Addgene_20160 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19031436
Proper citation: RRID:Addgene_20177 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4500; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19031436
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20176 Copy
Species: E. coli
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20214 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20213 Copy
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