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Species: Mus musculus
Genetic Insert: Galpha-o
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11802169
Comments: ApaI digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_15930 Copy
Species: Mus musculus
Genetic Insert: pLenti-CMV-TrkB (Ntrk2, Mouse)
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159402 Copy
Species: Mus musculus
Genetic Insert: gRNA library targeting mouse genes
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid #52961); Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32968282
Proper citation: RRID:Addgene_159392 Copy
Species: Mus musculus
Genetic Insert: M12
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSIIKS-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17662950
Comments: to make RNA probe, cut with SpeI. Then do in vitro transcription with T3RNA polymerase.
Proper citation: RRID:Addgene_15954 Copy
Species: Mus musculus
Genetic Insert: SRY (sex determining region Y)-box 2
Vector Backbone Description: Backbone Size:8263; Vector Backbone:pLOVE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371358
Comments: Addgene QC NGS analysis identifies an IS4-like element at nucleotides 6946-8283 in the plasmid backbone. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_15953 Copy
Species: Mus musculus
Genetic Insert: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Vector Backbone Description: Backbone Size:8263; Vector Backbone:pLOVE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371358
Proper citation: RRID:Addgene_15951 Copy
Species: Mus musculus
Genetic Insert: Kruppel-like factor 4
Vector Backbone Description: Backbone Size:8263; Vector Backbone:pLOVE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371358
Proper citation: RRID:Addgene_15950 Copy
Species: Mus musculus
Genetic Insert: M71
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSIISK-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17662950
Comments: to make RNA probe, cut with SacI. Then do in vitro transcription with T7RNA polymerase.
Proper citation: RRID:Addgene_15947 Copy
Species: Mus musculus
Genetic Insert: mDHFR
Vector Backbone Description: Vector Backbone:pGBKCg; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31337681
Comments: For 3' clonging site, a XhoI site that is present naturally in the sequence of a segment encoding the Gal4 DBD.
Proper citation: RRID:Addgene_159726 Copy
Species: Mus musculus
Genetic Insert: mDHFR
Vector Backbone Description: Vector Backbone:pGBKCg; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31337681
Comments: For 3' clonging site, a XhoI site that is present naturally in the sequence of a segment encoding the Gal4 DBD.
Proper citation: RRID:Addgene_159724 Copy
Species: Mus musculus
Genetic Insert: mDHFR
Vector Backbone Description: Vector Backbone:pGBKCg; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31337681
Comments: For 3' clonging site, a XhoI site that is present naturally in the sequence of a segment encoding the Gal4 DBD.
Proper citation: RRID:Addgene_159725 Copy
Species: Mus musculus
Genetic Insert: Nanog homeobox
Vector Backbone Description: Backbone Size:2229; Vector Backbone:pENTR1a; Vector Types:Mouse Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33091369
Comments: Please note that this plasmid runs as a multimer (>14kb). While this may reduce DNA yield, the plasmid still functions as expected for generating ES cells.
From original plasmid (Addgene 59995), 0.5kb of 5' and 3' homology arms were amplified with 2a-mCherry insert and cloned into pENTR backbone. A linker-venus sequence was inserted between the Nanog coding sequence and the 2a-mCherry cassette.
To target Nanog, we used a "double cut donor" strategy (see DOI 10.1186/s13059-017-1164-8). In brief, we designed an sgRNA sequence to make a double strand break near the stop codon of the Nanog ORF. We then inserted flanking sgRNA recognition sequences at the 5' and 3' ends of the Nanog targeting sequence in the repair template. Cells were co-transfected with pMP108 and the Cas9/sgRNA expression plasmid.
Proper citation: RRID:Addgene_159743 Copy
Species: Mus musculus
Genetic Insert: Htr2a
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159908 Copy
Species: Mus musculus
Genetic Insert: Netrin1
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159907 Copy
Species: Mus musculus
Genetic Insert: Kcnn3
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159904 Copy
Species: Mus musculus
Genetic Insert: Slc6a3
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159902 Copy
Species: Mus musculus
Genetic Insert: Kcnd3
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159912 Copy
Species: Mus musculus
Genetic Insert: Vamp2
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159920 Copy
Species: Mus musculus
Genetic Insert: AMPK gamma1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_15999 Copy
Species: Mus musculus
Genetic Insert: AMPK gamma1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15997 Copy
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