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Species: A. tumefaciens
Genetic Insert: mirA-3xFLAG
Vector Backbone Description: Backbone Size:6611; Vector Backbone:pRA301; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36645284
Comments: MirA is a cytoplasmic protein from Agrobacterium tumefaciens and can be used in conjunction with pJLG038 or pJLG039, which carry an inducible T7 polymerase. It can be replaced with a gene of interest for high yield protein expression from a native host.
Proper citation: RRID:Addgene_192979 Copy
Species: Zea mays (maize)
Genetic Insert: MNEAP1
Vector Backbone Description: Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33679862
Proper citation: RRID:Addgene_131021 Copy
Species: Zea mays (maize)
Genetic Insert: MKAKU41
Vector Backbone Description: Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33679862
Proper citation: RRID:Addgene_131015 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10
Vector Backbone Description: Vector Backbone:plCH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176871 Copy
Species: Synthetic
Genetic Insert: sgRNA_3
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176866 Copy
Species: Synthetic
Genetic Insert: sgRNA_5
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176869 Copy
Species: Synthetic
Genetic Insert: sgRNA_1
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176863 Copy
Species: Synthetic
Genetic Insert: sgRNA_2E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176865 Copy
Species: Synthetic
Genetic Insert: sgRNA_2
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176864 Copy
Species: Apis mellifera
Genetic Insert: Fragment of Def1 gene
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36460809
Proper citation: RRID:Addgene_183125 Copy
Species: Synthetic
Genetic Insert: ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178959 Copy
Species: Synthetic
Genetic Insert: ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178956 Copy
Species: Synthetic
Genetic Insert: SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178957 Copy
Species: Synthetic
Genetic Insert: hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178955 Copy
Species: Homo sapiens
Genetic Insert: YAP1 S94A
Vector Backbone Description: Vector Backbone:pDONR; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35170430
Proper citation: RRID:Addgene_184526 Copy
Species: Homo sapiens
Genetic Insert: YAP1 S127A
Vector Backbone Description: Vector Backbone:pDONR; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35170430
Proper citation: RRID:Addgene_184525 Copy
Species: Synthetic
Genetic Insert: Peroxisome signal peptide
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_202152 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: P49 terminator
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_202176 Copy
Species: Synthetic
Genetic Insert: evolved P450-T2 mutant gene and genes for styrene biosynthesis in S. albus
Vector Backbone Description: Vector Backbone:pAZA4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37138074
Proper citation: RRID:Addgene_207644 Copy
Species: Synthetic
Genetic Insert: SP-mCherry-GFP-HDEL
Vector Backbone Description: Backbone Marker:Ghent; Backbone Size:11628; Vector Backbone:pB7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33679862
Comments: Addgene QC NGS and diagnostic digest data shows a larger than expected plasmid sequence, relative to the sequence provided by the depositing laboratory, including a potential duplication of the insert sequence.
This construct is used as an positive control for apFRET in the ER or outer nuclear envelope in plant cells. The mCherry and GFP are fused by a GGSGG amino acid linker.
Proper citation: RRID:Addgene_159097 Copy
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