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Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164414 Copy
Species: Danio rerio
Genetic Insert: ythdf1
Vector Backbone Description: Vector Backbone:pSP64T; Vector Types:Production of mRNA for zebrafish injection.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378672
Proper citation: RRID:Addgene_164488 Copy
Species: Homo sapiens
Genetic Insert: WAF1 stop codon mut
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: PCR-generated cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16449 Copy
Species: Homo sapiens
Genetic Insert: WAF1 antisense
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: PCR-generated cDNA insert cloned into the NotI site of pCEP4.
pCEP-WAF1-AS is an antisense expression plasmid. Full-length WAF1 was cloned in opposite orientation in expression plasmid.
Proper citation: RRID:Addgene_16448 Copy
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164435 Copy
Species: Firefly
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164432 Copy
Species: Caenorhabditis elegans
Genetic Insert: C.elegans Tandem pore domain K+ channel twk-16
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pOX; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11301195
Proper citation: RRID:Addgene_16444 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2144057
Comments: The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
Proper citation: RRID:Addgene_16440 Copy
Vector Backbone Description: Backbone Size:11536; Vector Backbone:lentiCRISPRV2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33264619
Proper citation: RRID:Addgene_164273 Copy
Genetic Insert: SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164437 Copy
Species: Homo sapiens
Genetic Insert: WAF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: full-length WAF1 cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16450 Copy
Vector Backbone Description: Backbone Size:3726; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD123.102, Ligation number L4138.
Proper citation: RRID:Addgene_1645 Copy
Species: Mus musculus
Genetic Insert: Tgfb1
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32352930
Proper citation: RRID:Addgene_164427 Copy
Species: Mus musculus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036641
Proper citation: RRID:Addgene_164425 Copy
Species: Mus musculus
Genetic Insert: Tgfb3
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32352930
Proper citation: RRID:Addgene_164429 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164452 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164453 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
The Allen Institute for Brain Science recommends this plasmid as a potential substitute for Plasmid 163471: AiP1269-scAAV-mscRE4-minBGpromoter-SYFP2-WPRE3-bGHpA.
Proper citation: RRID:Addgene_164457 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164455 Copy
Genetic Insert: VSV-G
Vector Backbone Description: Vector Backbone:HDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164440 Copy
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