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Species: S. pyogenes Cas9, synthetic
Genetic Insert: Cas9-TagBFP with core cHS4 insulators
Vector Backbone Description: Vector Backbone:pPD008; Vector Types:Mammalian Expression, CRISPR, Cp36 donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41309578
Proper citation: RRID:Addgene_249153 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #17297; Vector Backbone:pLenti X1 Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41460725
Comments: Please visit https://www.biorxiv.org/content/10.1101/2025.02.21.639530v1 for bioRxiv preprint
Proper citation: RRID:Addgene_249282 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #211517; Vector Backbone:pGL4.R_TRE_minPro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41460725
Comments: Please visit https://www.biorxiv.org/content/10.1101/2025.02.21.639530v1 for bioRxiv preprint
Proper citation: RRID:Addgene_249281 Copy
Species: Homo sapiens
Genetic Insert: E2F2
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219013 Copy
Species: Homo sapiens
Genetic Insert: EGR2
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219014 Copy
Species: Homo sapiens
Genetic Insert: SOX2
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219392 Copy
Species: Homo sapiens
Genetic Insert: MAX
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219272 Copy
Species: Homo sapiens
Genetic Insert: Engineered IL15
Vector Backbone Description: Backbone Size:12064; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37363412
Comments: Engineered IL15 construct: IL15Ra signal sequence - IL15RA sushi domain - IL15RA hinge - linker - IL-15.
Proper citation: RRID:Addgene_248036 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG-tagged DR18
Vector Backbone Description: Backbone Size:12064; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37363412
Proper citation: RRID:Addgene_248037 Copy
Species: Homo sapiens
Genetic Insert: myc-tagged membrane bound IL2
Vector Backbone Description: Backbone Size:12121; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37363412
Comments: Membrane bound IL2 construct: IL2 signal sequence-IL2-Myc tag-IL2RA linker-IL2RB.
Proper citation: RRID:Addgene_248031 Copy
Species: Homo sapiens
Genetic Insert: myc-tagged membrane bound IL15
Vector Backbone Description: Backbone Size:12121; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37363412
Comments: Membrane bound IL15 construct: IgE signal peptide - IL15 - linker - IL15Ra - Myc.
Proper citation: RRID:Addgene_248032 Copy
Species: Homo sapiens
Genetic Insert: SOX6
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219274 Copy
Genetic Insert: AAV2-ITR-EGFP
Vector Backbone Description: Vector Backbone:pIDTsmart; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41479173
Proper citation: RRID:Addgene_240278 Copy
Genetic Insert: AAV2-ITR-EGFP
Vector Backbone Description: Vector Backbone:pIDTsmart; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41479173
Proper citation: RRID:Addgene_240279 Copy
Species: Homo sapiens
Genetic Insert: FOXP3
Vector Backbone Description: Backbone Size:6611; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219148 Copy
Species: Homo sapiens
Genetic Insert: SPIB
Vector Backbone Description: Backbone Size:6638; Vector Backbone:SFFV-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41539305
Comments: Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
Proper citation: RRID:Addgene_219320 Copy
Genetic Insert: AAV2-ITR-EGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41479173
Proper citation: RRID:Addgene_240280 Copy
Species: Synthetic
Genetic Insert: 2x Lyn Kinase membrane localization sequence
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:3948; Vector Backbone:pTwist Amp High Copy; Vector Types:zebrafish mRNA expression using SP6; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41426965
Comments: see https://www.fpbase.org/protein/mbaojin/
also, our mBaoJin sequence contains the n1 linker (MVSTGEELFTGVV) for fusion to the 2x Lyn kinase localization sequence
Proper citation: RRID:Addgene_247636 Copy
Species: Mus musculus
Genetic Insert: synaptophysin plus pHluorin
Vector Backbone Description: Backbone Marker:Kwang-Soo Kim Lab; Backbone Size:6324; Vector Backbone:pAAV2.5-THP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41348744
Proper citation: RRID:Addgene_242903 Copy
Species: Synthetic
Genetic Insert: (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
Vector Backbone Description: Backbone Size:8752; Vector Backbone:plenti.THP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41348744
Comments: For more info about iATPSnFR2 sensor: https://doi.org/10.1073/pnas.2314604121
Proper citation: RRID:Addgene_242904 Copy
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