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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS +; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36223382
Comments: U6 promoter from Anopheles gambiae AGAP013557 drives expression of a classical gRNA template (Jinek et al., 2012) in which a specific gRNA protospacer sequence can be cloned using two BbsI restrictions sites.
Proper citation: RRID:Addgene_173671 Copy
Species: Moloney murine leukemia virus
Genetic Insert: MMLV proviral DNA
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pBR322 modified to include SV40 origin; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10906179
Comments: pNCS contains an infectious copy of M-MuLV proviral DNA and carries a simian virus 40 replication origin to allow high-level expression in 293T cells. DPY-pNCS is similar to the wild-type parent but contains a deletion that removes 5 amino acids (DPPPY) from p12 region.
Proper citation: RRID:Addgene_17365 Copy
Species: Mus musculus
Genetic Insert: Wnt-1HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17367 Copy
Species: Homo sapiens
Genetic Insert: Wnt-2HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17368 Copy
Species: Phytophthora infestans
Genetic Insert: PITG_16705_T30-4
Vector Backbone Description: Backbone Marker:Youles SynBio plasmids; Backbone Size:8678; Vector Backbone:pICSL86977OD; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424903
Proper citation: RRID:Addgene_173550 Copy
Species: Homo sapiens
Genetic Insert: mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4396; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33453281
Proper citation: RRID:Addgene_173863 Copy
Species: Homo sapiens
Genetic Insert: mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3946; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33453281
Proper citation: RRID:Addgene_173865 Copy
Species: Homo sapiens
Genetic Insert: mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3946; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33453281
Proper citation: RRID:Addgene_173866 Copy
Species: Homo sapiens
Genetic Insert: mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3946; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33453281
Proper citation: RRID:Addgene_173867 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173747 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pwrt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173748 Copy
Species: Homo sapiens
Genetic Insert: AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4588; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23951027
Proper citation: RRID:Addgene_173861 Copy
Species: Homo sapiens
Genetic Insert: PLD4
Vector Backbone Description: Backbone Marker:Nemazee lab (Deli); Backbone Size:2000; Vector Backbone:SuExp; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:34620855
Proper citation: RRID:Addgene_173852 Copy
Species: Synthetic
Genetic Insert: EKARrEV-NLS
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pPBbsr2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Comments: Please note there are several ambiguous bases in EKARrEV. This is a highly repetitive region. Depositor is not concerned and ensures this plasmid is functional.
Proper citation: RRID:Addgene_173855 Copy
Species: Synthetic
Genetic Insert: mRFP-FKBP-mSos1-linkercat
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pT2Aneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173858 Copy
Species: Homo sapiens
Genetic Insert: PLD3
Vector Backbone Description: Backbone Marker:Nemazee lab (Deli); Backbone Size:2000; Vector Backbone:SuExp; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:34620855
Proper citation: RRID:Addgene_173851 Copy
Species: Homo sapiens
Genetic Insert: SERPiNA1
Vector Backbone Description: Vector Backbone:pET-8C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28752459
Comments: For details on mutating an internal initiation site in the SERPIINA1 gene (changing the pEAT8 plasmid to pEAT8-137) see: Laska, M. E. (2001) The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli, Thesis, Massachusetts Institute of Technology, Cambridge, MA
Proper citation: RRID:Addgene_173807 Copy
Species: Other
Genetic Insert: Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Vector Backbone Description: Vector Backbone:pET-23b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092728
Comments: Bacillus thuringiensis PI-PLC (UniProt P08954) cleaves GPI-anchored proteins off the surface of cells and can be used instead of the 97% identical, commercially available B. cereus enzyme (UniProt P14262).
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173808 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34190535
Comments: pIT5_TL is designed to be integrated into the lambda attachment site, using the lambda integrase helper plasmid pInt-ts (Addgene # 66076).
Proper citation: RRID:Addgene_173768 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34190535
Comments: pIT5_SL is designed to be integrated into the lambda attachment site, using the lambda integrase helper plasmid pInt-ts (Addgene # 66076).
Proper citation: RRID:Addgene_173769 Copy
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