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Species: Mus musculus
Genetic Insert: Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19109433
Proper citation: RRID:Addgene_20325 Copy
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: Mus musculus
Genetic Insert: cMYC
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:8376; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371436
Proper citation: RRID:Addgene_20324 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:8376; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371436
Proper citation: RRID:Addgene_20322 Copy
Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2764; Vector Backbone:pRset B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669
Proper citation: RRID:Addgene_20336 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20298 Copy
Species: Mus musculus
Genetic Insert: Klf4-F2A-Oct4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19109433
Proper citation: RRID:Addgene_20331 Copy
Species: A. victoria
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20296 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20299 Copy
Species: Mus musculus
Genetic Insert: Klf4-F2A-cMyc
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19109433
Proper citation: RRID:Addgene_20332 Copy
Genetic Insert: mir21(P) targets
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12554881
Proper citation: RRID:Addgene_20382 Copy
Species: E. coli
Genetic Insert: Pmyc1tetO-lacZ
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20315 Copy
Species: Mus musculus
Genetic Insert: Sirt6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15795229
Proper citation: RRID:Addgene_20275 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS1C
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20240 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_20368 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin B +1 transcript
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18796559
Proper citation: RRID:Addgene_20254 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19139065
Comments: TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
Proper citation: RRID:Addgene_20375 Copy
Species: human immunodeficiency virus type 1
Genetic Insert: HIV-1 protease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11502538
Proper citation: RRID:Addgene_20253 Copy
Species: Homo sapiens
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Size:6470; Vector Backbone:pMIR-REPORT; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18755897
Comments: There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function.
Proper citation: RRID:Addgene_20379 Copy
Species: Homo sapiens
Genetic Insert: CHKA
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2I7Q http://www.thesgc.org/structures/2I7Q/
PDB: 3G15 http://www.thesgc.org/structures/3G15/
PDB: 3F2R http://www.thesgc.org/structures/3F2R/
Proper citation: RRID:Addgene_25515 Copy
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