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Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173753 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173755 Copy
Species: Homo sapiens
Genetic Insert: Aβ(M1-42/C18C33)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pET vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35080857
Proper citation: RRID:Addgene_173759 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2::par-6::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173751 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173752 Copy
Species: Synthetic
Genetic Insert: STRHSTPAB
Vector Backbone Description: Vector Backbone:pST50Tr; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34408191
Proper citation: RRID:Addgene_173827 Copy
Species: Synthetic
Genetic Insert: STRHSTPABPACCBPv3
Vector Backbone Description: Vector Backbone:pST50Trc4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34408191
Proper citation: RRID:Addgene_173829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 2)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173786 Copy
Species: Rattus norvegicus
Genetic Insert: ZAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCDNA4 TO2 myc HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215647
Comments: The NZAP fragment was excised from pBabe-NZAP-Zeo with EcoRI-NotI and cloned into pCDNA4/TO2-myc-HisB to generate a myc-tagged
NZAP. The C-terminal portion of ZAP was cloned from a Rat2 cell cDNA library by PCR using sense primer CZAP-SP (5’-GAGCTCTCTGGGCTTAACC-3’) and antisense primer CZAP-AP (5’-
ATATAGGCGGCCGCCCTCTGGACCTCTTCTCTTC-3’). The sense primer lies
upstream from an internal NheI site in NZAP; the antisense primer introduces a NotI site immediately downstream from the coding sequence to facilitate its cloning into the myc-tagged expression vector.
Proper citation: RRID:Addgene_17381 Copy
Species: Rattus norvegicus
Genetic Insert: NZAP
Vector Backbone Description: Backbone Marker:Goff Lab; Backbone Size:0; Vector Backbone:pBabe-HAZ (modified pBabe vector); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215647
Proper citation: RRID:Addgene_17382 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34190535
Comments: pIT5_KP_LacI is designed to be integrated into the phi80 attachment site, using the phi80 integrase helper plasmid pAH123 (Addgene # 66077). This version of plasmid carries LacI repressor, and can be used for inducible gene expression (with IPTG) following integration. The pIT5_KP_LacI need to be propagated in a pir+ strain of E. coli.
Proper citation: RRID:Addgene_173780 Copy
Species: Mus musculus
Genetic Insert: Wnt-4HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.
Gavin BJ, McMahon JA, McMahon AP
In Genes Dev. 1990 Dec . 4(12B):2319-32.
Proper citation: RRID:Addgene_17375 Copy
Species: Other
Genetic Insert: HK022 Integrase
Vector Backbone Description: Backbone Marker:Haldimann and Wanner 2001; Vector Backbone:pAH69; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Proper citation: RRID:Addgene_173782 Copy
Species: Mus musculus
Genetic Insert: Wnt-6HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.
Gavin BJ, McMahon JA, McMahon AP
In Genes Dev. 1990 Dec . 4(12B):2319-32.
Proper citation: RRID:Addgene_17378 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pE-FLP; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Comments: Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.
The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_173785 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34190535
Comments: pIT5_KW is designed to be integrated into the Wphi attachment site, using the Whpi integrase helper plasmid pHelper_W.
Proper citation: RRID:Addgene_173776 Copy
Species: Other
Genetic Insert: Staphylococcus aureus plc
Vector Backbone Description: Vector Backbone:pET-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22390775
Comments: See UniProt ID P45723 for the mature, untagged sequence.
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173810 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34190535
Comments: pIT5_KH_VanR is designed to be integrated into the HK022 attachment site, using the HK022 integrase helper plasmid pAH69. This version of plasmid carries VanR repressor, and can be used for inducible gene expression (with Vanillic acid) following integration. The pIT5_KH_VanR need to be propagated in a pir+ strain of E. coli.
Proper citation: RRID:Addgene_173779 Copy
Genetic Insert: Myc-BirA-G3-ER
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pEntr; Vector Types:Gateway shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33888706
Comments: BirA-G3 sequence obtained from the Ting Lab:
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY. Efficient proximity labeling in living cells and organisms with TurboID. Nat Biotechnol. 2018 Oct;36(9):880-887. doi: 10.1038/nbt.4201. Epub 2018 Aug 20. Erratum in: Nat Biotechnol. 2020 Jan;38(1):108. PMID: 30125270; PMCID: PMC6126969.
Proper citation: RRID:Addgene_173814 Copy
Genetic Insert: Myc-BirA-G3-ER
Vector Backbone Description: Backbone Marker:Drosophila Genomics Resource Center; Vector Backbone:pWalium10-roe; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33888706
Comments: BirA-G3 sequence obtained from the Ting Lab:
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY. Efficient proximity labeling in living cells and organisms with TurboID. Nat Biotechnol. 2018 Oct;36(9):880-887. doi: 10.1038/nbt.4201. Epub 2018 Aug 20. Erratum in: Nat Biotechnol. 2020 Jan;38(1):108. PMID: 30125270; PMCID: PMC6126969.
Proper citation: RRID:Addgene_173815 Copy
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