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Species: Synthetic
Genetic Insert: PlacZ lambda cI-p38-alpha; PlacIq lacI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:ColEI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79223 Copy
Species: Homo sapiens
Genetic Insert: Vacuolar Protein Sorting 35 homolog
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2391; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:19553991
Proper citation: RRID:Addgene_21689 Copy
Species: Homo sapiens
Genetic Insert: Golgi Phosphoprotein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:897; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:19553991
Proper citation: RRID:Addgene_21688 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_187511 Copy
Species: Synthetic
Genetic Insert: B0030m
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_187502 Copy
Species: Arabidopsis thaliana
Genetic Insert: CDS mature HSP21 (44-227), Arabidopsis thaliana
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM1299; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172791 Copy
Species: Salmonella enterica NCTC10718
Genetic Insert: SeAvs1
Vector Backbone Description: Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188803 Copy
Species: Escherichia coli NCTC11132
Genetic Insert: EcAvs4
Vector Backbone Description: Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188806 Copy
Species: Corallococcus coralloides DSM2259
Genetic Insert: KpAvs4
Vector Backbone Description: Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188808 Copy
Vector Backbone Description: Vector Backbone:pBAtC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36456803
Proper citation: RRID:Addgene_189639 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190245 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190242 Copy
Genetic Insert: Mphot
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29885839
Proper citation: RRID:Addgene_186729 Copy
Genetic Insert: sgRNA-Target1
Vector Backbone Description: Backbone Size:17689; Vector Backbone:pMpGE010; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35476214
Proper citation: RRID:Addgene_186295 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190244 Copy
Species: E. coli
Genetic Insert: Retron Eco4 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35896746
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187201 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34124437
Comments: pSiMPls_N should be co-transformed with pSiMPls_C (or vice versa) in E. coli to get a reconstituted functional aminoglycoside adenylyltransferase thereby conferring resistance towards spectinomycin/streptomycin. The plasmids possess no additional antibiotic resistance gene.
Please see the Sequences link for the individual plasmid sequences.
An E. coli bacterial stab from Addgene will have both the plasmids pSiMPls_N + pSiMPls_C. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Proper citation: RRID:Addgene_167203 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-SpRY-UNG
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_174001 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_174002 Copy
Species: Discosoma sp.
Genetic Insert: red chromoprotein
Vector Backbone Description: Backbone Size:7023; Vector Backbone:pBTK402; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37225918
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190998 Copy
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