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Species: Homo sapiens
Genetic Insert: H2B-CFP
Vector Backbone Description: Backbone Size:6406; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Proper citation: RRID:Addgene_25998 Copy
Species: bacteriophage
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:6543; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Comments: Second AgeI site is introduced preventing AgeI-EcoRI shRNA subcloning. TRC library shRNAs can be subcloned as an AccI fragment.
Proper citation: RRID:Addgene_25997 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Size:6380; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Comments: Second AgeI site is introduced preventing AgeI-EcoRI shRNA subcloning. TRC library shRNAs can be subcloned as an AccI fragment or SphI-SacII fragment.
Proper citation: RRID:Addgene_25999 Copy
Species: Mus musculus
Genetic Insert: MyoD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Baculovirus Transfer Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15289617
Proper citation: RRID:Addgene_25994 Copy
Species: Mus musculus
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-)Myc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12527753
Comments: Though this vector backbone has Myc and His tags, they are not in frame with the Drp1. Do not use these tags for purification or detection after expression.
Proper citation: RRID:Addgene_26049 Copy
Genetic Insert: His-MBP-3C-lacZ
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26044 Copy
Genetic Insert: SS peptide-LacZ-KHHHHHH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26046 Copy
Genetic Insert: u-LacZ
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18662785
Proper citation: RRID:Addgene_26040 Copy
Genetic Insert: His-lacZ-KHHHHHH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: T7lacO, CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26043 Copy
Genetic Insert: His3C-lacZ
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: T7lacO, CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26042 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395539
Comments: The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX.
The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
Proper citation: RRID:Addgene_26061 Copy
Species: Homo sapiens
Genetic Insert: Cdc20
Vector Backbone Description: Backbone Marker:See note below; Backbone Size:4700; Vector Backbone:pVenus-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18997788
Comments: EYFP was swapped with Venus using AgeI and BsrGI restriction sites.
Proper citation: RRID:Addgene_26059 Copy
Species: Rattus norvegicus
Genetic Insert: Nav1.2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20543823
Proper citation: RRID:Addgene_26056 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11741955
Proper citation: RRID:Addgene_25976 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11741955
Proper citation: RRID:Addgene_25975 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15371409
Proper citation: RRID:Addgene_25978 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25972 Copy
Species: Danio rerio
Genetic Insert: fli1a intron 1
Vector Backbone Description: Backbone Size:2858; Vector Backbone:p5E; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23462469
Comments: "p5E" refers to 5' entry clones flanked by L4-R1
There are a few mismatches between depositor's sequence and Addgene's sequence. The changes should not affect plasmid function.
Proper citation: RRID:Addgene_26027 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25971 Copy
Genetic Insert: E1B basal promoter + UAS repeats
Vector Backbone Description: Backbone Size:2605; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: "p5E" refers to 5' entry clones flanked by L4-R1.
There are 2 mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches do not affect plasmid function.
Proper citation: RRID:Addgene_26026 Copy
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