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Species: Homo sapiens
Genetic Insert: GAlpha11-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5203; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140983 Copy
Species: Homo sapiens
Genetic Insert: GGamma1-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140989 Copy
Species: Homo sapiens
Genetic Insert: GAlpha12-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5203; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140985 Copy
Species: Homo sapiens
Genetic Insert: GAlpha13-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5203; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140986 Copy
Species: Homo sapiens
Genetic Insert: GGamma9-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140991 Copy
Species: Homo sapiens
Genetic Insert: Ago Chi-c
Vector Backbone Description: Backbone Size:5409; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16061186
Proper citation: RRID:Addgene_14086 Copy
Species: Homo sapiens
Genetic Insert: Human RhoC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:PCR-BluntII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10952316
Proper citation: RRID:Addgene_14099 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141112 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:PCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141113 Copy
Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Species: Homo sapiens
Genetic Insert: CMYB
Vector Backbone Description: Vector Backbone:pmiR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141114 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14104 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14102 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14103 Copy
Species: Homo sapiens
Genetic Insert: Antares-T2A-puromycin-EMCV IRES-SEPLuc
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4606; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Comments: Deletion in the luciferase does not affect the BRET efficiency of Antares
Proper citation: RRID:Addgene_141086 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Vector Backbone:pmiRA1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141121 Copy
Species: Homo sapiens
Genetic Insert: Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4606; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141089 Copy
Species: Homo sapiens
Genetic Insert: NFAT5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12080349
Comments: DNA binding domain mutant, dominant negative mutant. Also called DBD5.
Proper citation: RRID:Addgene_14112 Copy
Species: Homo sapiens
Genetic Insert: SEPLuc-E2A-cyOFP1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141084 Copy
Species: Homo sapiens
Genetic Insert: SEPLuc-EMCV IRES-Antares
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:5386; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141085 Copy
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