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Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/R160a_sgRNA2/R160a
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173756 Copy
Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/R160b_sgRNA2/160b
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173757 Copy
Species: S. tuberosum Group Phureja DM
Genetic Insert: Cas9_sgRNA1/390a_sgRNA2/390a
Vector Backbone Description: Backbone Size:15933; Vector Backbone:pDe_Cas9; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36072839
Comments: Spectinomycin is only for bacteria and Kanamycin is only for plants.
Proper citation: RRID:Addgene_173758 Copy
Species: Zea mays (maize)
Genetic Insert: MNEAP1
Vector Backbone Description: Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33679862
Proper citation: RRID:Addgene_131021 Copy
Species: Zea mays (maize)
Genetic Insert: MKAKU41
Vector Backbone Description: Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33679862
Proper citation: RRID:Addgene_131015 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10
Vector Backbone Description: Vector Backbone:plCH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176871 Copy
Species: Synthetic
Genetic Insert: sgRNA_3
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176866 Copy
Species: Synthetic
Genetic Insert: sgRNA_5
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176869 Copy
Species: Synthetic
Genetic Insert: sgRNA_1
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176863 Copy
Species: Synthetic
Genetic Insert: sgRNA_2E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176865 Copy
Species: Synthetic
Genetic Insert: sgRNA_2
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176864 Copy
Species: Apis mellifera
Genetic Insert: Fragment of Def1 gene
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36460809
Proper citation: RRID:Addgene_183125 Copy
Species: Synthetic
Genetic Insert: ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178959 Copy
Species: Synthetic
Genetic Insert: ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178956 Copy
Species: Synthetic
Genetic Insert: SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178957 Copy
Species: Synthetic
Genetic Insert: hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_178955 Copy
Species: Other
Genetic Insert: TTCG_Stop-tOCS_CGCT
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Backbone Size:2073; Vector Backbone:pAGM9121; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192351 Copy
Species: Arabidopsis thaliana
Genetic Insert: AATG_dttLbCas12a-i_TTCG
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Backbone Size:2073; Vector Backbone:pAGM3946; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36382936
Proper citation: RRID:Addgene_192350 Copy
Species: Other
Genetic Insert: Plac-RFP (IPTG-inducible red fluorescent protein)
Vector Backbone Description: Backbone Marker:Chain Biotech; Backbone Size:4759; Vector Backbone:pMTL82341; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36427328
Proper citation: RRID:Addgene_191346 Copy
Species: A. tumefaciens
Genetic Insert: pruR-3xFLAG
Vector Backbone Description: Backbone Size:6761; Vector Backbone:pRA301; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36645284
Comments: PruR is a periplasmic protein from Agrobacterium tumefaciens and can be used in conjunction with pJLG038 or pJLG039, which carry an inducible T7 polymerase. It can be replaced with a gene of interest for high yield protein expression from a native host.
Proper citation: RRID:Addgene_192980 Copy
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