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Species: Mus musculus
Genetic Insert: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33972728
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
Proper citation: RRID:Addgene_171693 Copy
Species: Mus musculus
Genetic Insert: ATP13A2
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31996848
Proper citation: RRID:Addgene_171771 Copy
Species: Mus musculus
Genetic Insert: SNX9
Vector Backbone Description: Vector Backbone:pLenti6 V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171943 Copy
Species: Mus musculus
Genetic Insert: ATP10B
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32172343
Proper citation: RRID:Addgene_171825 Copy
Species: Mus musculus
Genetic Insert: chromodomain helicase DNA binding protein 4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172099 Copy
Species: Mus musculus
Genetic Insert: Smad 3
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5129; Vector Backbone:SuperBackbone; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28320972
Comments: Human Smad3 and mouse Smad3 contain 100% sequence identity. The NG-Smad3 construct was placed downstream of a CMV promoter, and the mCerulean3 gene was fused with a 3× nuclear localization sequence (NLS) and placed downstream of an SV40 promoter.
Proper citation: RRID:Addgene_172098 Copy
Species: Mus musculus
Genetic Insert: Mouse PE sequence (mPE)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33458694
Proper citation: RRID:Addgene_172006 Copy
Species: Mus musculus
Genetic Insert: FoxO1 KQ
Vector Backbone Description: Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17681146
Proper citation: RRID:Addgene_66165 Copy
Species: Mus musculus
Genetic Insert: Pten deletion
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66588 Copy
Species: Mus musculus
Genetic Insert: Klf2
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27197208
Comments: The backbone of the plasmid is pCX-OKS-2A (Addgene plasmid #19771).
Proper citation: RRID:Addgene_66655 Copy
Species: Mus musculus
Genetic Insert: PDGFRalpha
Vector Backbone Description: Vector Backbone:pcDNA5FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26290382
Proper citation: RRID:Addgene_66789 Copy
Species: Mus musculus
Genetic Insert: PDGFRalpha
Vector Backbone Description: Vector Backbone:pcDNA5FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26290382
Proper citation: RRID:Addgene_66787 Copy
Species: Mus musculus
Genetic Insert: PDGFRbeta
Vector Backbone Description: Vector Backbone:pcDNA5FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26290382
Proper citation: RRID:Addgene_66790 Copy
Species: Mus musculus
Genetic Insert: IL-12B
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_66907 Copy
Species: Mus musculus
Genetic Insert: C/EBP alpha
Vector Backbone Description: Backbone Marker:Addgene Plasmid #18751; Backbone Size:6300; Vector Backbone:MSCV PIG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66985 Copy
Species: Mus musculus
Genetic Insert: Spi1
Vector Backbone Description: Backbone Marker:Addgene Plasmid #18751; Backbone Size:6300; Vector Backbone:MSCV PIG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66982 Copy
Species: Mus musculus
Genetic Insert: Erg (1-208)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66990 Copy
Species: Mus musculus
Genetic Insert: Erg (209-316)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66991 Copy
Species: Mus musculus
Genetic Insert: Sesn2
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203043
Proper citation: RRID:Addgene_66920 Copy
Species: Mus musculus
Genetic Insert: sgNT
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26178787
Comments: 20bp non-targeting sgRNA sequence obtained from Sanjana et al. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
Proper citation: RRID:Addgene_66895 Copy
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