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Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV.CAP-Mac VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37430038
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
This work was supported by funding from:
NIH BRAIN Armamentarium UF1MH128336
Michael J Fox Foundation for Parkinson's Research ASAP-020495
Proper citation: RRID:Addgene_200658 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204730 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204731 Copy
Species: Synthetic
Genetic Insert: ALFA tag
Vector Backbone Description: Backbone Size:3761; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201767 Copy
Species: Synthetic
Genetic Insert: ALFA tag
Vector Backbone Description: Backbone Size:3763; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201766 Copy
Species: Synthetic
Genetic Insert: NbALFA
Vector Backbone Description: Backbone Size:6086; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201769 Copy
Species: Synthetic
Genetic Insert: NbALFA
Vector Backbone Description: Backbone Size:6516; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201768 Copy
Species: Synthetic
Genetic Insert: iRFP713(C15S/V256C)
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198062 Copy
Species: Synthetic
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198060 Copy
Species: Synthetic
Genetic Insert: iRFP713(C15S/V256C)
Vector Backbone Description: Backbone Size:4425; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198069 Copy
Species: Synthetic
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:5186; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35545673
Proper citation: RRID:Addgene_204488 Copy
Species: Synthetic
Genetic Insert: PV1 VP1 protein with Histag
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction
Proper citation: RRID:Addgene_202071 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:vectorbuilder; Backbone Size:3970; Vector Backbone:pLV[Exp]-Neo-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36672454
Comments: Designed in Khan lab, manufactured by vectorbuilder.
Proper citation: RRID:Addgene_197459 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204724 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204728 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204726 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204729 Copy
Species: Synthetic
Genetic Insert: Hyperfolder YFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: Constitutive expression
Proper citation: RRID:Addgene_191201 Copy
Species: Synthetic
Genetic Insert: TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
Vector Backbone Description: Vector Backbone:pSBbi-GP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35618485
Proper citation: RRID:Addgene_186909 Copy
Species: Synthetic
Genetic Insert: VP2C-mRuby3
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: mRuby3 is slow to mature at 30 degC. Further incubation at 37 degC required after protein purification for complete fluorophore maturation.
Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182429 Copy
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