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pUCmini-iCAP-AAV.CAP-Mac
 
Resource Report
Resource Website 		RRID:Addgene_200658 Synthetic construct isolate AAV.CAP-Mac VP1 gene Synthetic Ampicillin PMID:37430038 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. This work was supported by funding from: NIH BRAIN Armamentarium UF1MH128336 Michael J Fox Foundation for Parkinson's Research ASAP-020495 Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 7 amino acid insertion after Q588 of the AAV9 CAP gene 2026-09-01 09:36:16 0
iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro
 
Resource Report
Resource Website 		RRID:Addgene_204730 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro
 
Resource Report
Resource Website 		RRID:Addgene_204731 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
pABY-T4
 
Resource Report
Resource Website 		RRID:Addgene_201767 ALFA tag Synthetic Ampicillin PMID:36190134 Backbone Size:3761; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:36:32 0
pABY-T3
 
Resource Report
Resource Website 		RRID:Addgene_201766 ALFA tag Synthetic Ampicillin PMID:36190134 Backbone Size:3763; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:36:32 0
pABY-c25
 
Resource Report
Resource Website 		RRID:Addgene_201769 NbALFA Synthetic Ampicillin PMID:36190134 Backbone Size:6086; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:36:32 0
pABY-c15
 
Resource Report
Resource Website 		RRID:Addgene_201768 NbALFA Synthetic Ampicillin PMID:36190134 Backbone Size:6516; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:36:32 0
pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_198062 iRFP713(C15S/V256C) Synthetic Ampicillin Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin C15S/V256C mutation 2026-09-01 09:35:33 0
pCSIIbleo-iRFP670-P2A-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_198060 iRFP670 Synthetic Ampicillin Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 09:35:33 0
pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_198069 iRFP713(C15S/V256C) Synthetic Ampicillin Backbone Size:4425; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C15S/V256C mutation 2026-09-01 09:35:34 0
pAAV-sL7-Cre-HA-WPRE
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_204488 Cre Synthetic Ampicillin PMID:35545673 Backbone Size:5186; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-01 09:37:16 1
pGEX-2T_PV1_VP1
 
Resource Report
Resource Website 		RRID:Addgene_202071 PV1 VP1 protein with Histag Synthetic Ampicillin PMID:37227147 Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:36:36 0
pDest Lenti CA-GFP
 
Resource Report
Resource Website 		RRID:Addgene_197459 GFP Synthetic Ampicillin PMID:36672454 Designed in Khan lab, manufactured by vectorbuilder. Backbone Marker:vectorbuilder; Backbone Size:3970; Vector Backbone:pLV[Exp]-Neo-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin S65T 2026-09-01 09:35:23 0
iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP
 
Resource Report
Resource Website 		RRID:Addgene_204724 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
iF58 PB-Zim3-dCas9-mScarlet-Puro
 
Resource Report
Resource Website 		RRID:Addgene_204728 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
iF53 PB-Zim3-dCas9-mScarlet-Hygro
 
Resource Report
Resource Website 		RRID:Addgene_204726 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
 
Resource Report
Resource Website 		RRID:Addgene_204729 Zim3-dCas9 Synthetic Ampicillin Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:37:20 0
pAAV-CAG-hfYFP
 
Resource Report
Resource Website 		RRID:Addgene_191201 Hyperfolder YFP Synthetic Ampicillin PMID:36344833 Constitutive expression Backbone Marker:Scott Sternson; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105Y/K149N/T167I/H203Y/K206V/D234N 2026-09-01 09:33:39 0
InterCatch-3xL
 
Resource Report
Resource Website 		RRID:Addgene_186909 TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10 Synthetic Ampicillin PMID:35618485 Vector Backbone:pSBbi-GP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:32:37 0
mRuby3 only VLP
 
Resource Report
Resource Website 		RRID:Addgene_182429 VP2C-mRuby3 Synthetic Ampicillin mRuby3 is slow to mature at 30 degC. Further incubation at 37 degC required after protein purification for complete fluorophore maturation. Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-01 09:31:23 0

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