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Species: Mus musculus
Genetic Insert: Ig germline mu switch region
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6278424
Comments: Includes a 5 kb EcoRI/BamHI fragment with Smu (3 kb deletion relative to the germline) and both 5' and 3' flanking sequences.
Proper citation: RRID:Addgene_25912 Copy
Species: A. victoria
Genetic Insert: EYFP H148Q
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11423120
Proper citation: RRID:Addgene_25873 Copy
Vector Backbone Description: Backbone Marker:Ivanova, NB; Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371338
Comments: Empty Vector pFUGW-H1.
The H1 promoter was cloned into the PacI site of FUGW (Addgene Plasmid# 14883). Please see cloning protocol for recommended shRNA subcloning procedure.
Proper citation: RRID:Addgene_25870 Copy
Species: Homo sapiens
Genetic Insert: RapR-FAK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20581846
Proper citation: RRID:Addgene_25928 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20581846
Comments: FRB is a small domain derived from full length mTOR. In brief, rapamycin binds to a protein called FKBP12. Rapamycin bound to FKBP12 inhibits mTOR signaling
Proper citation: RRID:Addgene_25920 Copy
Species: Mus musculus
Genetic Insert: Brahma related gene 1
Vector Backbone Description: Backbone Marker:Toshio Kitamura; Backbone Size:4628; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20550931
Proper citation: RRID:Addgene_25855 Copy
Species: Mus musculus
Genetic Insert: Brg1 associated factor 155
Vector Backbone Description: Backbone Size:4628; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20550931
Proper citation: RRID:Addgene_25856 Copy
Species: Mus musculus
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Ivanova, NB; Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371338
Comments: This plasmid targets the open reading frame of p21. The 19mer target sequence is: TTAGGACTCAACCGTAATA
Proper citation: RRID:Addgene_25868 Copy
Genetic Insert: hsa-miR-34a promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Comments: The sequence of the promoter region of miR-34a corresponds to the one described by Raver-Shapira et al. Mol Cell. 2007;26:731–743.
Proper citation: RRID:Addgene_25799 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-150
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_25792 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-34a
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_25791 Copy
Species: Homo sapiens
Genetic Insert: shRNA against human MYB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6400; Vector Backbone:pSIREN-RetroQ; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_25790 Copy
Species: Homo sapiens
Genetic Insert: human Cryptochrome 1
Vector Backbone Description: Backbone Size:5274; Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_25843 Copy
Species: Homo sapiens
Genetic Insert: human Cryptochrome 2
Vector Backbone Description: Backbone Size:5274; Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12627958
Proper citation: RRID:Addgene_25842 Copy
Species: Homo sapiens
Genetic Insert: RapR-p38
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20581846
Comments: Note that the Addgene quality control sequence does not match the author-supplied sequence exactly. There is NO PstI site at the end of the insert.
Proper citation: RRID:Addgene_25935 Copy
Species: Aq. Victoria and Discosoma sp
Genetic Insert: ClopHensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5363; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20581829
Proper citation: RRID:Addgene_25938 Copy
Species: Mus musculus
Genetic Insert: RapR-Src
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20581846
Proper citation: RRID:Addgene_25934 Copy
Species: Homo sapiens
Genetic Insert: pWPI_SPbFGF
Vector Backbone Description: Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17728358
Proper citation: RRID:Addgene_25812 Copy
Species: Mus musculus
Genetic Insert: RapR-Src
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20581846
Proper citation: RRID:Addgene_25933 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21706014
Comments: This is a control entry vector. There is no Stop codon.
Proper citation: RRID:Addgene_25894 Copy
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