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Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19883615
Proper citation: RRID:Addgene_25968 Copy
Species: Homo sapiens
Genetic Insert: B-Myb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10593981
Proper citation: RRID:Addgene_25965 Copy
Genetic Insert: Vinculin tension sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613844
Comments: Note that this vector lacks the SP6 sequence present in pcDNA3.1. There is a small insertion in the vector sequence, upstream of Vinculin. Plasmid function should not be affected.
Proper citation: RRID:Addgene_26019 Copy
Species: Mus musculus
Genetic Insert: B-Myb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11733503
Proper citation: RRID:Addgene_25967 Copy
Species: Mus musculus
Genetic Insert: B-Myb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10593981
Proper citation: RRID:Addgene_25966 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25960 Copy
Genetic Insert: N terminal Strep tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26015 Copy
Genetic Insert: N terminal VSV tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26018 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25962 Copy
Genetic Insert: N terminal V5 tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26017 Copy
Genetic Insert: N terminal HSV tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26012 Copy
Genetic Insert: N terminal HA tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26011 Copy
Genetic Insert: N terminal Flag tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26010 Copy
Vector Backbone Description: Backbone Size:3875; Vector Backbone:pBTBX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20148414
Comments: Addgene identified an additional Thr on the C-terminal of the Rep protein compared to the author's sequence. This discrepancy was found in similar plasmids from this deposit.
Proper citation: RRID:Addgene_26067 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Marker:See note below; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20412769
Comments: The backbone was derived from pECFP-N1. ECFP was replaced with mCherry.
There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.
Proper citation: RRID:Addgene_26063 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Marker:See note below; Backbone Size:4500; Vector Backbone:pVenus-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pVenus-N1 was derived from pEYFP vector from Clontech.
pVenus-N1 contains no Pst site
Proper citation: RRID:Addgene_26062 Copy
Genetic Insert: Cdk1 FRET Sensor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20412769
Comments: Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.
Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG
Proper citation: RRID:Addgene_26064 Copy
Species: Homo sapiens
Genetic Insert: CD11b promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3199; Vector Backbone:pGEM3zf-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7811988
Comments: The cloned CD11b promoter in this construct contains some 5'UTR, including the transcription start site.
Proper citation: RRID:Addgene_26168 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:modified pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17694064
Comments: This plasmid is to be used ONLY as a negative control for the CMV-d2eGFP plasmids in the associated paper. The multiple cloning site has been destroyed and should not be used for further cloning.
Proper citation: RRID:Addgene_26164 Copy
Species: Homo sapiens
Genetic Insert: RAB25:HPC038-B06:C25773
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2OIL
http://www.thesgc.org/structures/2OIL/
Proper citation: RRID:Addgene_26163 Copy
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