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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
CCNT1_pLX307
 
Resource Report
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1+ mentions
RRID:Addgene_98328 CCNT1 Homo sapiens Ampicillin PMID:27684187 Addgene's sequencing results found H519R, S566P, and P712S mutations compared to NCBI reference sequence NP_001231.2. These mutations are not known to affect CCNT1 function. Backbone Marker:David Root (Addgene plasmid # 41392); Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin H519R, S566P, and P712S 2026-09-01 10:05:38 1
lentiCRISPRv2 hygro
 
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50+ mentions
RRID:Addgene_98291 P2A-hygro Ampicillin PMID:30894629 Double stranded oligonucleotides encoding sgRNA sequences can be cloned between the BsmBI restriction sites as for lentiCRISPRv2. The second MluI site in the original lentiCRISPRv2 plasmid was destroyed by Klenow end-filling and religation after MluI digestion. For target guide (sgRNA) sequence cloning instructions, please see https://www.addgene.org/52961 for the Zhang's lab lentiCRISPR v2 guide. Backbone Marker:Feng Zhang (Addgene #52961); Backbone Size:15261; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 84
lentiCRISPRv2 puro
 
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100+ mentions
RRID:Addgene_98290 P2A-puro Ampicillin PMID:30894629 P2A-antibiotic resistance gene cassettes from the plasmids in this deposit (https://www.addgene.org/depositing/74286/) can be cloned in place of the P2A-puro gene using the unique BamHI and MluI sites. The second MluI site in the original lentiCRISPRv2 plasmid was destroyed by Klenow end-filling and religation after MluI digestion. For target guide (sgRNA) sequence cloning instructions, please see https://www.addgene.org/52961 for the Zhang's lab lentiCRISPR v2 guide. Backbone Marker:Feng Zhang (Addgene #52961); Backbone Size:14877; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 256
lentiCRISPRv2 neo
 
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10+ mentions
RRID:Addgene_98292 P2a-neo Ampicillin PMID:30894629 Double stranded oligonucleotides encoding sgRNA sequences can be cloned between the BsmBI restriction sites as for lentiCRISPRv2. The second MluI site in the original lentiCRISPRv2 plasmid was destroyed by Klenow end-filling and religation after MluI digestion. For target guide (sgRNA) sequence cloning instructions, please see https://www.addgene.org/52961 for the Zhang's lab lentiCRISPR v2 guide. Backbone Marker:Feng Zhang (Addgene #52961); Backbone Size:15072; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 29
FERMT1_pLX307
 
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1+ mentions
RRID:Addgene_98333 FERMT1 Homo sapiens Ampicillin PMID:27684187 Backbone Marker:David Root (Addgene plasmid # 41392); Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 1
pIMVAu1
 
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1+ mentions
RRID:Addgene_98301 HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292) Other Ampicillin PMID:28239415 Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence. Vector Backbone:pRS423; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 1
pIMVAd39T
 
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1+ mentions
RRID:Addgene_98303 pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1 Saccharomyces cerevisiae Ampicillin PMID:28239415 Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence. Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:38 1
pTNFR1-tdEOS
 
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1+ mentions
RRID:Addgene_98273 TNFR1 Homo sapiens Ampicillin PMID:22749881 fusion protein: TNFR1-tdEosfp Backbone Marker:MoBiTec; Vector Backbone:pcDNA3-Flag1-tdEosFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:37 2
BRD7 (BRD7A-c005)
 
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1+ mentions
RRID:Addgene_98245 BRD7A Homo sapiens Kanamycin Use BL21(DE3)-R3-pRARE2 for expression. T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. 5MQ1: http://www.thesgc.org/structures/5MQ1 Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin pfam00439:Bromodomain 136-223 2026-09-01 10:05:37 2
TCF7L2_pLX307
 
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1+ mentions
RRID:Addgene_98373 TCF7L2 Homo sapiens Ampicillin PMID:27684187 Backbone Marker:David Root (Addgene plasmid # 41392); Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Isoform X25 2026-09-01 10:05:39 1
ZNF217_pLX307
 
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1+ mentions
RRID:Addgene_98384 ZNF217 Homo sapiens Ampicillin PMID:27684187 Backbone Marker:David Root (Addgene plasmid # 41392); Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:39 1
pEN_TT 3xFLAG ID2
 
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1+ mentions
RRID:Addgene_98390 ID2 Homo sapiens Gentamicin PMID:29161592 Gateway entry vector for expression of 3xFLAG ID2 (human) Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin 2026-09-01 10:05:39 1
pEN_TT 3xFLAG Luciferase
 
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1+ mentions
RRID:Addgene_98391 Luciferase Other Gentamicin PMID:29161592 Gateway entry vector for expression of 3xFLAG Luciferase Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin 2026-09-01 10:05:39 1
pSLIK TT 3xFLAG ID2 hygro
 
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1+ mentions
RRID:Addgene_98394 ID2 Homo sapiens Ampicillin PMID:29161592 Lentiviral mammalian expression plasmid for inducible expression of 3xFLAG ID2 with hygromycin selection; recombined from Addgene #98390 and Addgene #25737. Backbone Marker:Addgene #25737; Backbone Size:13286; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:39 2
pBabe NEO Venus
 
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1+ mentions
RRID:Addgene_98396 Venus Other Ampicillin PMID:29161592 Retroviral vector for Venus expression Backbone Marker:Addgene #1767; Vector Backbone:pBABE-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:39 1
pLKO-Tet-On-shRNA-Control
 
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10+ mentions
RRID:Addgene_98398 non-targeting randomized sequence Homo sapiens Ampicillin PMID:34650052 Backbone Marker:Dmitri Wiederschain ; Backbone Size:8758; Vector Backbone:Tet-pLKO-Puro (pLKO-Tet-On) Addgene #21915; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:39 10
pQE60 Trx1 SSAAA
 
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1+ mentions
RRID:Addgene_98403 Trx1 SSAAA Homo sapiens Ampicillin PMID:17557078 Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C32S, C35S, C62A, C69A, C73A 2026-09-01 10:05:39 1
DHL708
 
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1+ mentions
RRID:Addgene_98417 Genotype: MC4100 ∆clpPX Streptomycin PMID:27732583 Strain DHL708 was built by deleting the clpPX operon with lambda-Red mediated homologous recombination. The FRT-flanked Kan cassette was then flipped out using the FLP recombinase (pCP20). The deletion region can be PCR-amplified and sequenced using the primers CCGCTCGAGTTTACGCAGCATAACGCGCTAAATTC and CGTCAGTATATGGGGATGTTTCCCC. Originally described in Landgraf, D., Okumus, B., Chien, P., Baker, T. A. & Paulsson, J. Segregation of molecules at cell division reveals native protein localization. Nat Methods 9, 480–482 (2012). Vector Backbone:none; Vector Types:; Bacterial Resistance:Streptomycin 2026-09-01 10:05:40 1
pCML 220
 
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1+ mentions
RRID:Addgene_98588 Kanamycin PMID:25080893 Backbone Size:3100; Vector Backbone:Derived from pDMSD-Y2 (Kostecki et al., 2010, 10.1371/journal.pone.0009225); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 10:05:41 1
p46Cpf1-OP2
 
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1+ mentions
RRID:Addgene_98592 FnCpf1 Francisella Chloramphenicol PMID:30356638 Backbone Marker:Datsenko KA & Wanner BL (PMID:10829079); Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol Codon optimized for E. coli 2026-09-01 10:05:41 3

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