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Genetic Insert: C terminal VSV tag
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26181 Copy
Genetic Insert: C terminal HA tag
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26174 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-NF-YB
Vector Backbone Description: Backbone Size:2650; Vector Backbone:pPR244; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26147 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-elav1
Vector Backbone Description: Backbone Size:2650; Vector Backbone:pPR244; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26143 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-hnRNPA2
Vector Backbone Description: Backbone Size:2650; Vector Backbone:pPR244; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26145 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-eIF3c
Vector Backbone Description: Backbone Size:2650; Vector Backbone:pPR244; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26142 Copy
Species: Mycobacterium marinum + Photorhabdus luminescens
Genetic Insert: G13 promoter + Bacterial luciferase operon
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pMV306hsp; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20520722
Comments: The sequence of the Lux operon is theoretical. As such, there are a few differences between Addgene sequence and full plasmid sequence. These differences do not affect plasmid function.
Qazi, S.N., et al., agr expression precedes escape of internalized Staphylococcus aureus from the host endosome. Infect Immun, 2001. 69(11): p. 7074-82.
Proper citation: RRID:Addgene_26160 Copy
Species: Mycobacterium bovis BCG
Genetic Insert: hsp60 promoter
Vector Backbone Description: Backbone Size:3938; Vector Backbone:pMV306; Vector Types:Mycobacteria integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20520722
Comments: There are a few sequence discrepancies between author sequence and Addgene quality control sequence for the hsp promoter. The hsp promoter sequence was taken from the pSMT3 vector and is theoretical sequence. These differences do not affect function.
Garbe, T.R., et al., Transformation of mycobacterial species using hygromycin resistance as selectable marker. Microbiology, 1994. 140 ( Pt 1): p. 133-8.
Proper citation: RRID:Addgene_26155 Copy
Species: Photinus pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pMV306hsp; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20520722
Proper citation: RRID:Addgene_26156 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-sped-1
Vector Backbone Description: Backbone Size:2650; Vector Backbone:pPR244; Vector Types:RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26151 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-eIF3c
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26153 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 2 alpha
Vector Backbone Description: Backbone Size:5140; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086860
Proper citation: RRID:Addgene_26091 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php
Proper citation: RRID:Addgene_26094 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-PABPC
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26129 Copy
Species: trans-activator - KRAB
Genetic Insert: tTR-KRAB
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pSin-EF2-Nanog; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Proper citation: RRID:Addgene_26122 Copy
Species: Rattus norvegicus
Genetic Insert: Synaptophysin GCaMP2
Vector Backbone Description: Backbone Size:3957; Vector Backbone:pEGFP-N1 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19898484
Comments: ORF frame 3 (624-2915): Rat SyGCaMP2.
Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
Proper citation: RRID:Addgene_26124 Copy
Species: Aquorea victoria
Genetic Insert: Precursor mT/mG
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCA-HZ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26123 Copy
Species: Homo sapiens
Genetic Insert: Grb2 P49L
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSVE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8479536
Comments: mutagenesis performed using oligo GGA AAA GAC GGC TTC ATT TTA AAG AAC TAC ATA GAA ATG, which creates a new DraI site and mutates codon 49 from P to L.
Proper citation: RRID:Addgene_26085 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-sped-1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20844018
Proper citation: RRID:Addgene_26138 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The pET28-MHL vector (GenBank accession EF456735) was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an 18 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site. The GSS residues after the Met start site were removed to reduce N-terminal gluconoylation via preventing N-terminal Met excision. Two stop codons are included in the vector at the C-terminal cloning site.
Insertion of DNA sequence into the cloning/expression region is performed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.
http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php
Proper citation: RRID:Addgene_26096 Copy
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