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Vector Backbone Description: Backbone Size:2839; Vector Backbone:BB3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98549 Copy
Species: Homo sapiens
Genetic Insert: RNA polymerase II C-terminal domain
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28945358
Proper citation: RRID:Addgene_98678 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29358076
Proper citation: RRID:Addgene_98657 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26455390
Proper citation: RRID:Addgene_98656 Copy
Species: Mus musculus
Genetic Insert: EGFPL10a
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5601; Vector Backbone:pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423326
Proper citation: RRID:Addgene_98747 Copy
Species: Homo sapiens
Genetic Insert: miR302/367 (miRNA cluster)
Vector Backbone Description: Vector Backbone:pSimpleII; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26584543
Comments: Use in addition to episomal reprogramming vectors
Proper citation: RRID:Addgene_98748 Copy
Species: Synthetic
Genetic Insert: 3xNLS
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_98875 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_98877 Copy
Species: Homo sapiens
Genetic Insert: PRDX5
Vector Backbone Description: Vector Backbone:pLD-puro-CcVA (Addgene plasmid #24588); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29128334
Comments: The C terminus VA tag (C terminal on the tagged protein) consists of the following features: Beacon tag [ELFNLLGENQPPVVIK] - 2XStrepII - 6XHis - 2XTEV - 3XFlag
Proper citation: RRID:Addgene_98688 Copy
Species: Homo sapiens
Genetic Insert: PRDX5
Vector Backbone Description: Vector Backbone:pLD-puro-CcVA (Addgene plasmid #24588); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29128334
Comments: The C terminus VA tag (C terminal on the tagged protein) consists of the following features: Beacon tag [ELFNLLGENQPPVVIK] - 2XStrepII - 6XHis - 2XTEV - 3XFlag
Proper citation: RRID:Addgene_98689 Copy
Species: Homo sapiens
Genetic Insert: PRDX5
Vector Backbone Description: Backbone Size:2815; Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29128334
Proper citation: RRID:Addgene_98692 Copy
Species: Homo sapiens
Genetic Insert: PRDX5
Vector Backbone Description: Backbone Size:2815; Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29128334
Proper citation: RRID:Addgene_98694 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98699 Copy
Species: Synthetic
Genetic Insert: Lck
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98822 Copy
Species: Synthetic
Genetic Insert: Lck
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98821 Copy
Species: Synthetic
Genetic Insert: EB3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98826 Copy
Species: Synthetic
Genetic Insert: Lamp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98828 Copy
Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Addgene #25890; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of human PC5A (PCSK5) mutant T288P with C-terminal V5 tag.
Wild-type PCSK5 sequence was mutated with QuikChange Site Directed Mutagenesis.
Proper citation: RRID:Addgene_98709 Copy
Species: Synthetic
Genetic Insert: ITPKA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98829 Copy
Species: Synthetic
Genetic Insert: CytERM
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing found an E382Q amino acid residue substitution in the LaminB insert. The affect of this residue change is not known.
Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98831 Copy
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