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Species: Homo sapiens
Genetic Insert: TH1534
Vector Backbone Description: Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37134173
Comments: Class: F, Family: Frizzled. Ensembl ID: ENSG00000111432, Uniprot ID: Q9ULW2
Proper citation: RRID:Addgene_213246 Copy
Species: Homo sapiens
Genetic Insert: MeCP2
Vector Backbone Description: Backbone Marker:Promega Corporation; Vector Backbone:pHT2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35156529
Proper citation: RRID:Addgene_212033 Copy
Species: Homo sapiens
Genetic Insert: CENP-T(1-242)-I3-01
Vector Backbone Description: Backbone Marker:Cheeseman Lab; Vector Backbone:pNM280; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211866 Copy
Species: Homo sapiens
Genetic Insert: TH1532
Vector Backbone Description: Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37134173
Comments: Class: F, Family: Frizzled. Ensembl ID: ENSG00000163251, Uniprot ID: Q13467
Proper citation: RRID:Addgene_213244 Copy
Species: Homo sapiens
Genetic Insert: TH1533
Vector Backbone Description: Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37134173
Comments: Class: F, Family: Frizzled. Ensembl ID: ENSG00000164930, Uniprot ID: O60353
Proper citation: RRID:Addgene_213245 Copy
Species: Homo sapiens
Genetic Insert: Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
Vector Backbone Description: Backbone Marker:Invitrogen (modified); Backbone Size:9879; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Comments: This vector is similar to pLD401 (Taylor et al. Cell 2013) but the C-terminal tag on ORF2p is simpler.
The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).
Proper citation: RRID:Addgene_213026 Copy
Species: Homo sapiens
Genetic Insert: GRM4
Vector Backbone Description: Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37134173
Comments: Class: C, Family: Metabotropic Glutamate. Ensembl ID: ENSG00000124493, Uniprot ID: Q14833
Proper citation: RRID:Addgene_213302 Copy
Species: Homo sapiens
Genetic Insert: Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
Vector Backbone Description: Backbone Marker:Invitrogen (modified); Backbone Size:9879; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Comments: The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).
Proper citation: RRID:Addgene_213028 Copy
Species: Homo sapiens
Genetic Insert: Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
Vector Backbone Description: Backbone Marker:Invitrogen (modified); Backbone Size:9807; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Comments: The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).
Proper citation: RRID:Addgene_213029 Copy
Species: Homo sapiens
Genetic Insert: LINE-1 ORF2p (1-1275)
Vector Backbone Description: Backbone Marker:Kacper Rogala, modified by Martin Taylor; Backbone Size:3541; Vector Backbone:pDARMO-PolH2.1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Comments: Includes polyhedrin terminator and backbone is designed to be useful in MultiBac scenarios. Note that relative to some insect vectors, the Tn7 sites are reversed, so the insert may be flipped in the destination bacmid and PCR primers may need to be swapped accordingly.
The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).
For more on pDARMO vectors see Rogala, K. B. et al. Structural basis for the docking of mTORC1 on the lysosomal surface. Science 366, 468-475 (2019). https://doi.org/10.1126/science.aay0166
Proper citation: RRID:Addgene_213025 Copy
Species: Homo sapiens
Genetic Insert: CENP-T[1-242/3D]-I3-01
Vector Backbone Description: Vector Backbone:pRSETa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211890 Copy
Species: Homo sapiens
Genetic Insert: mTagBFP2-GABARAP(G116A)-GABARAP
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40395516
Proper citation: RRID:Addgene_212109 Copy
Species: Homo sapiens
Genetic Insert: GABARAP(G116A)-mTagBFP2-GABARAP(G116A)
Vector Backbone Description: Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40395516
Proper citation: RRID:Addgene_212108 Copy
Species: Homo sapiens
Genetic Insert: Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
Vector Backbone Description: Backbone Marker:Invitrogen (modified); Backbone Size:9994; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Proper citation: RRID:Addgene_213030 Copy
Species: Homo sapiens
Genetic Insert: GABARAP(5X/G116A)-mTagBFP2-GABARAP
Vector Backbone Description: Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40395516
Proper citation: RRID:Addgene_212107 Copy
Species: Homo sapiens
Genetic Insert: Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
Vector Backbone Description: Backbone Marker:Genscript / Invitrogen; Backbone Size:8859; Vector Backbone:pCEP Puro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096902
Comments: The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).
For integration with Sleeping Beauty such as SB100x (Izsvak lab, Addgene #34879). This vector is similar to pYX056 from An lab, see also:
Xie, Y. et al. Cell division promotes efficient retrotransposition in a stable L1 reporter cell line. Mob DNA 4, 10 (2013). https://doi.org/10.1186/1759-8753-4-10
and
Xie, Y., Rosser, J. M., Thompson, T. L., Boeke, J. D. & An, W. Characterization of L1 retrotransposition with high-throughput dual-luciferase assays. Nucleic Acids Res 39, e16 (2011). https://doi.org/10.1093/nar/gkq1076
Proper citation: RRID:Addgene_213031 Copy
Species: Homo sapiens
Genetic Insert: UCK2 D62A
Vector Backbone Description: Backbone Size:5623; Vector Backbone:pMXS-IRES-BLAST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39592843
Proper citation: RRID:Addgene_211530 Copy
Species: Homo sapiens
Genetic Insert: hP53DD
Vector Backbone Description: Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39737975
Proper citation: RRID:Addgene_214082 Copy
Species: Homo sapiens
Genetic Insert: PEmax-P2A-hP53DD
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39737975
Proper citation: RRID:Addgene_214084 Copy
Species: Homo sapiens
Genetic Insert: Split-KRas fragment fused with FRB
Vector Backbone Description: Backbone Marker:Genephore; Backbone Size:5695; Vector Backbone:pMAL-c5X-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39058298
Proper citation: RRID:Addgene_214302 Copy
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