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Vector Backbone Description: Backbone Size:9377; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21706014
Comments: There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25890 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21706014
Comments: This is a control entry vector. There is no Stop codon.
Proper citation: RRID:Addgene_25893 Copy
Species: Homo sapiens
Genetic Insert: shRNA against human CDK6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6400; Vector Backbone:pSIREN-RetroQ; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_25789 Copy
Species: Aq. Victoria and Discosoma sp
Genetic Insert: PalmPalm-ClopHensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5344; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20581829
Proper citation: RRID:Addgene_25940 Copy
Species: Homo sapiens
Genetic Insert: H2B-CFP
Vector Backbone Description: Backbone Size:6406; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Proper citation: RRID:Addgene_25998 Copy
Species: bacteriophage
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:6543; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Comments: Second AgeI site is introduced preventing AgeI-EcoRI shRNA subcloning. TRC library shRNAs can be subcloned as an AccI fragment.
Proper citation: RRID:Addgene_25997 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Size:6380; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20526348
Comments: Second AgeI site is introduced preventing AgeI-EcoRI shRNA subcloning. TRC library shRNAs can be subcloned as an AccI fragment or SphI-SacII fragment.
Proper citation: RRID:Addgene_25999 Copy
Species: Mus musculus
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-)Myc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12527753
Comments: Though this vector backbone has Myc and His tags, they are not in frame with the Drp1. Do not use these tags for purification or detection after expression.
Proper citation: RRID:Addgene_26049 Copy
Genetic Insert: His-MBP-3C-lacZ
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26044 Copy
Genetic Insert: SS peptide-LacZ-KHHHHHH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26046 Copy
Genetic Insert: His-lacZ-KHHHHHH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: T7lacO, CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26043 Copy
Genetic Insert: His3C-lacZ
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5457; Vector Backbone:pTriEx-2; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17317681
Comments: Promoters/baculoviral recombination sites: T7lacO, CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
Proper citation: RRID:Addgene_26042 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395539
Comments: The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX.
The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
Proper citation: RRID:Addgene_26061 Copy
Species: Rattus norvegicus
Genetic Insert: Nav1.2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20543823
Proper citation: RRID:Addgene_26056 Copy
Species: Mus musculus
Genetic Insert: L-Myc
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:6340; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660764
Proper citation: RRID:Addgene_26023 Copy
Species: Homo sapiens
Genetic Insert: L-Myc
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:6340; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660764
Proper citation: RRID:Addgene_26022 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25970 Copy
Genetic Insert: Tension Sensor module
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613844
Comments: The are a few discrepancies with the author sequence. THese mismatches are in the pcDNA3.1(+) part of the vector, not the specific inserts. So they should be of little consequence.
Proper citation: RRID:Addgene_26021 Copy
Species: Homo sapiens
Genetic Insert: tumor protein p53
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5385; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11034215
Comments: There is a L383H mutation in the sequence. The mutation should not affect plasmid function.
Proper citation: RRID:Addgene_25989 Copy
Species: Homo sapiens
Genetic Insert: ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
Vector Backbone Description: Backbone Marker:James Thomson; Backbone Size:6500; Vector Backbone:pSIN-EF2-MCS-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19670287
Comments: Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).
Proper citation: RRID:Addgene_25983 Copy
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