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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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TH1534-DuET Resource Report Resource Website |
RRID:Addgene_213246 | TH1534 | Homo sapiens | Ampicillin | PMID:37134173 | Class: F, Family: Frizzled. Ensembl ID: ENSG00000111432, Uniprot ID: Q9ULW2 | Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | |
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pGhMeCP2 (pc3972) Resource Report Resource Website |
RRID:Addgene_212033 | MeCP2 | Homo sapiens | Kanamycin | PMID:35156529 | Backbone Marker:Promega Corporation; Vector Backbone:pHT2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:20:39 | 0 | ||
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pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor Resource Report Resource Website |
RRID:Addgene_211866 | CENP-T(1-242)-I3-01 | Homo sapiens | Ampicillin | Backbone Marker:Cheeseman Lab; Vector Backbone:pNM280; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | N/A | 2026-08-29 01:20:39 | 0 | ||
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TH1532-DuET Resource Report Resource Website |
RRID:Addgene_213244 | TH1532 | Homo sapiens | Ampicillin | PMID:37134173 | Class: F, Family: Frizzled. Ensembl ID: ENSG00000163251, Uniprot ID: Q13467 | Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | |
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TH1533-DuET Resource Report Resource Website |
RRID:Addgene_213245 | TH1533 | Homo sapiens | Ampicillin | PMID:37134173 | Class: F, Family: Frizzled. Ensembl ID: ENSG00000164930, Uniprot ID: O60353 | Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:45 | 0 | |
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pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro Resource Report Resource Website |
RRID:Addgene_213026 | Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag | Homo sapiens | Ampicillin | PMID:38096902 | This vector is similar to pLD401 (Taylor et al. Cell 2013) but the C-terminal tag on ORF2p is simpler. The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). | Backbone Marker:Invitrogen (modified); Backbone Size:9879; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | |
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GRM4-DuET Resource Report Resource Website |
RRID:Addgene_213302 | GRM4 | Homo sapiens | Ampicillin | PMID:37134173 | Class: C, Family: Metabotropic Glutamate. Ensembl ID: ENSG00000124493, Uniprot ID: Q14833 | Vector Backbone:pcDNA 3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | |
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pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646) Resource Report Resource Website |
RRID:Addgene_213028 | Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag | Homo sapiens | Ampicillin | PMID:38096902 | The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). | Backbone Marker:Invitrogen (modified); Backbone Size:9879; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | E43S D145N double mutant EN- endonuclease catalytic dead | 2026-08-29 01:20:39 | 0 |
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pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646) Resource Report Resource Website |
RRID:Addgene_213029 | Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag | Homo sapiens | Ampicillin | PMID:38096902 | The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). | Backbone Marker:Invitrogen (modified); Backbone Size:9807; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:45 | 0 | |
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pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1 Resource Report Resource Website |
RRID:Addgene_213025 | LINE-1 ORF2p (1-1275) | Homo sapiens | Ampicillin | PMID:38096902 | Includes polyhedrin terminator and backbone is designed to be useful in MultiBac scenarios. Note that relative to some insect vectors, the Tn7 sites are reversed, so the insert may be flipped in the destination bacmid and PCR primers may need to be swapped accordingly. The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). For more on pDARMO vectors see Rogala, K. B. et al. Structural basis for the docking of mTORC1 on the lysosomal surface. Science 366, 468-475 (2019). https://doi.org/10.1126/science.aay0166 | Backbone Marker:Kacper Rogala, modified by Martin Taylor; Backbone Size:3541; Vector Backbone:pDARMO-PolH2.1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:45 | 0 | |
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pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01 Resource Report Resource Website |
RRID:Addgene_211890 | CENP-T[1-242/3D]-I3-01 | Homo sapiens | Ampicillin | Vector Backbone:pRSETa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T11D, T27D, T85D | 2026-08-29 01:20:39 | 0 | ||
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pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP Resource Report Resource Website |
RRID:Addgene_212109 | mTagBFP2-GABARAP(G116A)-GABARAP | Homo sapiens | Ampicillin | PMID:40395516 | Backbone Marker:Thermo Fisher; Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | ||
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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A) Resource Report Resource Website |
RRID:Addgene_212108 | GABARAP(G116A)-mTagBFP2-GABARAP(G116A) | Homo sapiens | Ampicillin | PMID:40395516 | Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | G116A (lipidation deficiency) of both GABARAPs | 2026-08-29 01:20:39 | 0 | |
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pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro Resource Report Resource Website |
RRID:Addgene_213030 | Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag | Homo sapiens | Ampicillin | PMID:38096902 | Backbone Marker:Invitrogen (modified); Backbone Size:9994; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | ||
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pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP Resource Report Resource Website |
RRID:Addgene_212107 | GABARAP(5X/G116A)-mTagBFP2-GABARAP | Homo sapiens | Ampicillin | PMID:40395516 | Backbone Size:5049; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 5X: K2A, K13A, R15A, K20A, K23A mutations of upstream GABARAP, G116A mutation of upstream GABARAP | 2026-08-29 01:20:44 | 0 | |
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pRT006.3 L1 dual-luciferase AI retrotransposition reporter Resource Report Resource Website |
RRID:Addgene_213031 | Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker | Homo sapiens | Ampicillin | PMID:38096902 | The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). For integration with Sleeping Beauty such as SB100x (Izsvak lab, Addgene #34879). This vector is similar to pYX056 from An lab, see also: Xie, Y. et al. Cell division promotes efficient retrotransposition in a stable L1 reporter cell line. Mob DNA 4, 10 (2013). https://doi.org/10.1186/1759-8753-4-10 and Xie, Y., Rosser, J. M., Thompson, T. L., Boeke, J. D. & An, W. Characterization of L1 retrotransposition with high-throughput dual-luciferase assays. Nucleic Acids Res 39, e16 (2011). https://doi.org/10.1093/nar/gkq1076 | Backbone Marker:Genscript / Invitrogen; Backbone Size:8859; Vector Backbone:pCEP Puro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:39 | 0 | |
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pMXS-IRES-Blast coUCK2 D62A Resource Report Resource Website |
RRID:Addgene_211530 | UCK2 D62A | Homo sapiens | Ampicillin | PMID:39592843 | Backbone Size:5623; Vector Backbone:pMXS-IRES-BLAST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | D62A (catalytic mutant) | 2026-08-29 01:20:44 | 0 | |
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EF1a-hP53DD (TET+CTD) Resource Report Resource Website |
RRID:Addgene_214082 | hP53DD | Homo sapiens | Ampicillin | PMID:39737975 | Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:47 | 0 | ||
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pCMV-PEmax-P2A-hP53DD Resource Report Resource Website |
RRID:Addgene_214084 | PEmax-P2A-hP53DD | Homo sapiens | Ampicillin | PMID:39737975 | Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:41 | 0 | ||
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pMAL-MBP-FRB-KRas/N12 Resource Report Resource Website |
RRID:Addgene_214302 | Split-KRas fragment fused with FRB | Homo sapiens | Ampicillin | PMID:39058298 | Backbone Marker:Genephore; Backbone Size:5695; Vector Backbone:pMAL-c5X-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:20:42 | 0 |
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